Document Detail


Elimination of polarity in the carotenoid terminus promotes the exposure of B850-binding sites (Tyr 44, 45) and ANS-mediated energy transfer in LH2 complexes of Rhodobacter sphaeroides.
MedLine Citation:
PMID:  15530435     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Carotenoids in the peripheral light-harvesting complexes (LH2) of the green mutant (GM309) of Rhodobacter sphaeroides were identified as containing neurosporenes, which lack the polar CH(3)O group, compared to spheroidenes in native-LH2 of R. sphaeroides 601. After LH2 complexes were treated with 1-anilino-8-naphthalene sulfonate (ANS), new energy transfer pathways from ANS or tryptophan to carotenoids were discovered in both native- and GM309-LH2. The carotenoid fluorescence intensity of GM309-LH2 was greater than that of native-LH2 when bound with ANS, suggesting that the elimination of polarity in the neurosporene increases the energy transfer from ANS to carotenoid. The fact that two alpha-tyrosines (alpha-Tyr 44, 45, B850-binding sites) in each alpha-apoprotein of GM309-LH2 were more easily modified than those of native-LH2 by N-acetylimidazole (NAI) indicates that the elimination of polarity in the neurosporene terminus increases the exposure of these sites to solution.
Authors:
Yuan Liu; Yongqiang Wu; Chunhe Xu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  325     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2004 Dec 
Date Detail:
Created Date:  2004-11-08     Completed Date:  2005-02-17     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  600-4     Citation Subset:  IM    
Affiliation:
Shanghai Institute of Plant Physiology, Shanghai institute for Biological Sciences, Chinese Academy of Science, Shanghai 200032, PR China.
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MeSH Terms
Descriptor/Qualifier:
Anilino Naphthalenesulfonates / chemistry
Binding Sites
Carotenoids / chemistry*,  genetics,  metabolism*
Chromatography, High Pressure Liquid / methods
Energy Transfer
Imidazoles / chemistry
Light-Harvesting Protein Complexes / chemistry,  metabolism*
Mass Spectrometry / methods
Mutation
Phenotype
Rhodobacter sphaeroides / metabolism*
Spectrometry, Fluorescence
Tryptophan / chemistry
Tyrosine / metabolism*
Chemical
Reg. No./Substance:
0/Anilino Naphthalenesulfonates; 0/Imidazoles; 0/Light-Harvesting Protein Complexes; 2466-76-4/N-acetylimidazole; 36-88-4/Carotenoids; 55520-40-6/Tyrosine; 73-22-3/Tryptophan; 82-76-8/1-anilino-8-naphthalenesulfonate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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