Document Detail


Elimination of mitochondrial elements and improved viability in hybrid cells.
MedLine Citation:
PMID:  7221838     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Experiments were carried out to determine whether the mitochondria-specific dye rhodamine-6G (R6G) can affect transmission of cytoplasmic determinants in mammalian cells. When one parental cell type was treated with R6G prior to fusion with an untreated partner, the subsequent hybridization frequencies in both intra- and interspecific crosses were not adversely affected, even though R6G was extremely toxic to the parental cells. In addition, cells lethally treated with R6G could be rescued by fusion with cytoplasm alone from untreated cells. When chloramphenicol (CAP) resistant cells were used as the R6G-treated parent, the expression of CAP resistance in hybrids and cybrids was greatly reduced. Thus R6G can be used to control the input of cytoplasmic determinants into fused cells. In the interspecific (Chinese hamster x mouse) crosses, it was also seen that the majority of hybrids which had not been R6G pretreated grew poorly or degenerated after a short time. In contrast, nearly all hybrids in crosses where the hamster parent was R6G pretreated grew vigorously. The concomitant elimination of inviability and loss of mitochondrial determinants in R6G-pretreated hybrids suggests that interactions involving mitochondrial gene products or components can influence growth characteristics in interspecific somatic cell hybrids.
Authors:
M L Ziegler; R L Davidson
Related Documents :
18076988 - Induction of trophoblast cell fusion by a protein tyrosine phosphatase inhibitor.
15831748 - Unveiling the mechanisms of cell-cell fusion.
19222348 - Transcriptional changes in somatic cells recovered from embryonic stem-somatic heteroka...
19301948 - Effects of disulfide bridges glycoprotein e1 on fusogenic activity of rubella virus.
17363068 - Isolation of outer hair cells from the cochlear sensory epithelium in whole-mount prepa...
12166678 - Increased pigment and lipid content, lipid variety, and cell and population size of the...
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Somatic cell genetics     Volume:  7     ISSN:  0098-0366     ISO Abbreviation:  Somatic Cell Genet.     Publication Date:  1981 Jan 
Date Detail:
Created Date:  1981-06-23     Completed Date:  1981-06-23     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  7506054     Medline TA:  Somatic Cell Genet     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  73-88     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Cell Fusion
Cell Line
Cell Survival / drug effects*
Cricetinae
Cricetulus
DNA, Mitochondrial / genetics*
Extrachromosomal Inheritance / drug effects*
Hybrid Cells / physiology*
Kidney
Lung
Mice
Mice, Inbred BALB C
Rhodamines / pharmacology*
Xanthenes / pharmacology*
Grant Support
ID/Acronym/Agency:
HD 04807/HD/NICHD NIH HHS; HD 06276/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/DNA, Mitochondrial; 0/Rhodamines; 0/Xanthenes; 989-38-8/rhodamine 6G

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Quantitative cell fusion: derivation and application of theoretical models.
Next Document:  A prospective evaluation of indirect methods for detecting carotid atherosclerosis.