| Elimination of hypoxic protection by 5-thio-D-glucose in multicell spheroids. | |
| | |
MedLine Citation:
|
PMID: 719634 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The effect of 5-thio-D-glucose on oxic and hypoxic V79-171 Chinese hamster cells was studied in vitro with single cells and multicell spheroids. At concentrations that were not toxic to oxic cells, this compound killed hypoxic cells with a D0 of 1 hr with a 5 mM concentration and more rapidly at 10 mM beginning 2 hr after incubation at 37 degrees. 5-Thio-D-glucose also sensitized hypoxic cells to radiation and protected oxic cells from radiation damage. Multicell spheroids irradiated after incubation with the compound demonstrated increased radiosensitivity, although the relative contribution of cytotoxicity and hypoxic cell sensitization could not be evaluated. Spheroid reoxygenation by decreased cell respiration was determined not to be a contributing factor, suggesting that the spheroid-sensitizing effect was due to drug effects on hypoxic cells. The dramatic increase in multicell spheroid radiosensitivity that resulted from treatment with 5-thio-D-glucose suggests that this compound may be used to increase the effectiveness of radiotherapy by eliminating hypoxic protection. |
| | |
Authors:
|
C W Song; J J Clement; S H Levitt |
Related Documents
:
|
3285204 - Interaction between cigarette smoke condensate and radiation for the induction of genot... 2587514 - Induction of dna-protein crosslinks in melanotic cloudman s91 mouse melanoma cells and ... 17899084 - Further mechanistic unravelling of the influence of the cell cycle effects on the radio... 11771544 - The role of promotion in carcinogenesis from protracted high-let exposure. 19892764 - From spores to antibiotics via the cell cycle. 22745314 - Graded levels of pax2a and pax8 regulate cell differentiation during sensory placode fo... |
Publication Detail:
|
Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: Cancer research Volume: 38 ISSN: 0008-5472 ISO Abbreviation: Cancer Res. Publication Date: 1978 Dec |
Date Detail:
|
Created Date: 1979-02-12 Completed Date: 1979-02-12 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 2984705R Medline TA: Cancer Res Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 4499-503 Citation Subset: IM; S |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Anoxia* Cell Survival / drug effects*, radiation effects Cells, Cultured Glucose / analogs & derivatives*, pharmacology Oxygen* Oxygen Consumption / drug effects Radiation-Sensitizing Agents |
| Chemical | |
Reg. No./Substance:
|
0/Radiation-Sensitizing Agents; 50-99-7/Glucose; 7782-44-7/Oxygen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Enhancement of liver microsome epoxide hydratase activity in rodents by treatment with 2(3)-tert-but...
Next Document: Destruction of regional lymph node metastases of rat mammary adenocarcinoma 13762A by treatment with...