| Elg1, an alternative subunit of the RFC clamp loader, preferentially interacts with SUMOylated PCNA. | |
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MedLine Citation:
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PMID: 20571511 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Replication-factor C (RFC) is a protein complex that loads the processivity clamp PCNA onto DNA. Elg1 is a conserved protein with homology to the largest subunit of RFC, but its function remained enigmatic. Here, we show that yeast Elg1 interacts physically and genetically with PCNA, in a manner that depends on PCNA modification, and exhibits preferential affinity for SUMOylated PCNA. This interaction is mediated by three small ubiquitin-like modifier (SUMO)-interacting motifs and a PCNA-interacting protein box close to the N-terminus of Elg1. These motifs are important for the ability of Elg1 to maintain genomic stability. SUMOylated PCNA is known to recruit the helicase Srs2, and in the absence of Elg1, Srs2 and SUMOylated PCNA accumulate on chromatin. Strains carrying mutations in both ELG1 and SRS2 exhibit a synthetic fitness defect that depends on PCNA modification. Our results underscore the importance of Elg1, Srs2 and SUMOylated PCNA in the maintenance of genomic stability. |
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Authors:
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Oren Parnas; Adi Zipin-Roitman; Boris Pfander; Batia Liefshitz; Yuval Mazor; Shay Ben-Aroya; Stefan Jentsch; Martin Kupiec |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-06-22 |
Journal Detail:
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Title: The EMBO journal Volume: 29 ISSN: 1460-2075 ISO Abbreviation: EMBO J. Publication Date: 2010 Aug |
Date Detail:
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Created Date: 2010-08-04 Completed Date: 2010-08-26 Revised Date: 2011-08-25 |
Medline Journal Info:
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Nlm Unique ID: 8208664 Medline TA: EMBO J Country: England |
Other Details:
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Languages: eng Pagination: 2611-22 Citation Subset: IM |
Affiliation:
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Department of Molecular Microbiology and Biotechnology, Tel Aviv University, Ramat Aviv, Israel. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Antigens, Nuclear / chemistry, genetics, metabolism* Carrier Proteins / genetics, metabolism* DNA Helicases / genetics, metabolism Gene Deletion Genomic Instability* Molecular Sequence Data Protein Binding Saccharomyces cerevisiae / genetics, metabolism* Saccharomyces cerevisiae Proteins / chemistry, genetics, metabolism* Sequence Alignment Small Ubiquitin-Related Modifier Proteins / chemistry, metabolism* Ubiquitination |
| Chemical | |
Reg. No./Substance:
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0/Antigens, Nuclear; 0/Carrier Proteins; 0/Elg1 protein, S cerevisiae; 0/POL30 protein, S cerevisiae; 0/Saccharomyces cerevisiae Proteins; 0/Small Ubiquitin-Related Modifier Proteins; 125481-05-2/HPR5 protein, S cerevisiae; EC 3.6.1.-/DNA Helicases |
| Comments/Corrections | |
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