| Elevation of transcription factor Islet-1 levels in vivo increases β-cell function but not β-cell mass. | |
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MedLine Citation:
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PMID: 22595886 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A decrease in the expression of Islet-1 (Isl-1), an islet transcription factor, has been reported in several physiological settings of reduced β-cell function. Here, we investigate whether an increased level of Isl-1 in islet cells can enhance β-cell function and/or mass. We demonstrate that transgenic mice with Isl-1 overexpression display improved glucose tolerance and enhanced insulin secretion without significant changes in β cell mass. From our microarray study, we identify approximately 135 differentially expressed genes in the islets of Isl-1 overexpressing mice that have been implicated to function in numerous biological processes including protein trafficking, metabolism and differentiation. Using real-time PCR we have confirmed upregulation of Caps2, Sec14l4, Slc2a10, P2rx7, Afamin, and Neurogenin 3 that may in part mediate the observed improved insulin secretion in Isl-1 overexpressing mice. These findings show for the first time that Isl-1 is a key factor in regulating adult β cell function in vivo, and suggest that Isl-1 elevation could be beneficial to improve glucose homeostasis. |
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Authors:
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Jingxuan Liu; Erik R Walp; Catherine Lee May |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2012-05-01 |
Journal Detail:
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Title: Islets Volume: 4 ISSN: 1938-2022 ISO Abbreviation: Islets Publication Date: 2012 May-Jun |
Date Detail:
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Created Date: 2012-07-31 Completed Date: 2013-03-08 Revised Date: 2013-05-20 |
Medline Journal Info:
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Nlm Unique ID: 101495366 Medline TA: Islets Country: United States |
Other Details:
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Languages: eng Pagination: 199-206 Citation Subset: IM |
Affiliation:
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Department of Pathology and Laboratory Medicine, Children's Hospital of Philadelphia, Philadelphia, PA, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Diabetes Mellitus / metabolism, pathology Gene Expression Profiling Gene Expression Regulation Glucose / metabolism Insulin / metabolism Insulin-Secreting Cells / cytology, metabolism, physiology* LIM-Homeodomain Proteins / metabolism* Male Mice Mice, Inbred C57BL Mice, Transgenic Oligonucleotide Array Sequence Analysis RNA / chemistry, genetics Real-Time Polymerase Chain Reaction Transcription Factors / metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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DK078606/DK/NIDDK NIH HHS; P30-DK050306/DK/NIDDK NIH HHS; P30-DK19525/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Insulin; 0/LIM-Homeodomain Proteins; 0/Transcription Factors; 0/insulin gene enhancer binding protein Isl-1; 50-99-7/Glucose; 63231-63-0/RNA |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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