Document Detail


Elevation of hepatic metallothionein in rats chronically exposed to dietary ethionine.
MedLine Citation:
PMID:  4035706     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Following 30 weeks of exposure to 0.3% ethionine in the diet, male Fischer rats bearing liver cell tumors showed a marked increase (9.5 fold) in hepatic metallothionein (MT) concentrations relative to control, as assessed by the cadmium-hemoglobin assay. To confirm the presence of MT in the tumor-containing livers of ethionine-fed rats, biochemical analyses were performed. Spectral analysis of a purified protein from ethionine-treated livers revealed a UV absorption spectrum characteristic of MT. Gel filtration (Sephadex G-75) indicated a prominent copper (Cu) and zinc (Zn)-containing peak with a Mr (approx. 9 000) similar to that of MT. Concurrent with the elevation in hepatic MT in ethionine-fed rats there was an increase in the concentration of Cu and Zn in hepatic cytosol.
Authors:
M P Waalkes
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Toxicology letters     Volume:  26     ISSN:  0378-4274     ISO Abbreviation:  Toxicol. Lett.     Publication Date:  1985 Aug 
Date Detail:
Created Date:  1985-10-17     Completed Date:  1985-10-17     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  7709027     Medline TA:  Toxicol Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  133-8     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Chromatography, Gel
Copper / metabolism
Diet
Ethionine / toxicity*
Liver / drug effects*,  metabolism
Male
Metallothionein / metabolism*
Rats
Rats, Inbred F344
Spectrophotometry, Atomic
Spectrophotometry, Ultraviolet
Time Factors
Zinc / metabolism
Chemical
Reg. No./Substance:
13073-35-3/Ethionine; 7440-50-8/Copper; 7440-66-6/Zinc; 9038-94-2/Metallothionein

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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