| Elevation of cytosolic calcium is sufficient to induce growth inhibition in a B cell lymphoma. | |
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MedLine Citation:
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PMID: 7691606 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Recently, we have described that anti-IgM antibodies profoundly inhibited the growth of BKS-2, an immature B cell lymphoma. In this report, we demonstrated that ionomycin alone at very low concentrations (20 nM) inhibited the growth of BKS-2 cells completely. The levels of intracellular Ca2+ induced by the inhibitory concentrations of ionomycin were comparable to those in anti-IgM-treated cells. The growth inhibition caused by ionomycin was reversed by phorbol 12-myristate 13-acetate and lipopolysaccharide. In addition, the immunosuppressants, cyclosporin A and FK506 conferred significant protection from the negative signal induced by ionomycin. However, either cyclosporin A, FK506 or lipopolysaccharide was not found to have direct effect on ionomycin-induced Ca2+ mobilization in BKS-2 cells. Also, ionomycin augmented the anti-IgM-induced growth arrest in these cells. Furthermore, BKS-2 cells that were exposed to anti-IgM or ionomycin underwent apoptosis as characterized by DNA fragmentation. Thus, the characteristics of growth inhibition induced by ionomycin and anti-IgM appeared to be similar in that phorbol 12-myristate 13-acetate, lipopolysaccharide, cyclosporin A and FK506 caused significant reversal from such negative signals and both ionomycin and anti-IgM induced apoptosis in these cells. Altogether, these results showed that the elevation of intracellular Ca2+ alone was sufficient to inhibit the growth of some B lymphoma cells. |
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Authors:
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S Muthukkumar; V Udhayakumar; S Bondada |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: European journal of immunology Volume: 23 ISSN: 0014-2980 ISO Abbreviation: Eur. J. Immunol. Publication Date: 1993 Oct |
Date Detail:
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Created Date: 1993-11-12 Completed Date: 1993-11-12 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 1273201 Medline TA: Eur J Immunol Country: GERMANY |
Other Details:
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Languages: eng Pagination: 2419-26 Citation Subset: IM |
Affiliation:
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Department of Microbiology and Immunology, University of Kentucky, Lexington 40536-0230. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antibodies, Anti-Idiotypic / pharmacology Calcium / metabolism* Cell Division / drug effects Cyclosporine / pharmacology Cytosol / metabolism Immunoglobulin M Ionomycin / pharmacology Lipopolysaccharides / pharmacology Lymphoma, B-Cell / drug therapy, metabolism*, pathology Mice Mice, Inbred CBA Tacrolimus / pharmacology Tetradecanoylphorbol Acetate / pharmacology |
| Grant Support | |
ID/Acronym/Agency:
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AG 05731/AG/NIA NIH HHS; AI 21490/AI/NIAID NIH HHS; K04 AG 00422/AG/NIA NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Anti-Idiotypic; 0/Immunoglobulin M; 0/Lipopolysaccharides; 109581-93-3/Tacrolimus; 16561-29-8/Tetradecanoylphorbol Acetate; 56092-81-0/Ionomycin; 59865-13-3/Cyclosporine; 7440-70-2/Calcium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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