Document Detail


Elevation by atrial natriuretic factors of cyclic GMP levels in astroglia-rich cultures from murine brain.
MedLine Citation:
PMID:  2463339     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Atrial natriuretic factors, peptide hormones originally found in the heart, slowly but strongly elevate the level of cyclic GMP in primary astrocyte-rich cultures derived from brains of newborn rats or mice but not in neuron-rich cultures prepared from embryonic rat brain. In the absence of a phosphodiesterase inhibitor, a plateau level of cyclic GMP is obtained within 10 min. In the presence of the inhibitor 3-isobutyl-1-methylxanthine, the concentration of cyclic GMP continues to rise, even after 30 min. The elevation of the level of cyclic GMP in response to atrial natriuretic factor is much more pronounced in the rat cultures than the mouse cultures. Even at peptide concentrations of 1 microM, plateaus of the concentration-response curves are not yet reached. The potencies of the active peptides vary over a range of approximately 1.5 orders of magnitude, with atriopeptins II and III and auriculin A being the most potent ones. These results suggest (a) that atrial natriuretic factors may regulate functions of glial cells, most likely of astrocytes, in brain and (b) that such cultures may be useful tools in defining such astroglial functions.
Authors:
A Friedl; C Harmening; F Schmalz; B Schuricht; M Schiller; B Hamprecht
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of neurochemistry     Volume:  52     ISSN:  0022-3042     ISO Abbreviation:  J. Neurochem.     Publication Date:  1989 Feb 
Date Detail:
Created Date:  1989-02-23     Completed Date:  1989-02-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985190R     Medline TA:  J Neurochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  589-97     Citation Subset:  IM    
Affiliation:
Physiologisch-chemisches Institut der Universität, Tübingen, F.R.G.
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MeSH Terms
Descriptor/Qualifier:
1-Methyl-3-isobutylxanthine / pharmacology
Animals
Astrocytes / drug effects,  metabolism*
Atrial Natriuretic Factor / pharmacology*
Brain / drug effects,  metabolism*
Cells, Cultured
Cyclic GMP / biosynthesis*
Kinetics
Mice
Neurons / drug effects,  metabolism
Peptide Fragments / pharmacology
Rats
Rats, Inbred Strains
Chemical
Reg. No./Substance:
0/Peptide Fragments; 28822-58-4/1-Methyl-3-isobutylxanthine; 7665-99-8/Cyclic GMP; 85637-73-6/Atrial Natriuretic Factor; 91421-87-3/atrial natriuretic factor prohormone (8-33)

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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