| Elevation of anions in exercise-induced acidosis: a study by ion-exchange chromatography/mass spectrometry. | |
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MedLine Citation:
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PMID: 17939163 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Acidosis is a major factor that determines the upper limit of exercise endurance. We have previously shown that anions usually associated with intermediary metabolism are elevated in critically ill patients with metabolic acidosis and contribute significantly to acidosis generation. This study was to determine whether volunteers with normal metabolism would exhibit similar elevations in anions associated with intermediate metabolism when exposed to a short-term physiological stress leading to a brief lactic acidosis. Physiological stress was induced on five healthy male subjects by means of a ramped exercise protocol. Blood was obtained immediately prior to and post-exercise, plasma ultrafiltrate was prepared and analysed immediately both by enzyme assay and liquid chromatography coupled to electrospray-mass spectrometry (LC/ESI-MS). Metabolic acidosis concomitant with a significant increase in blood lactate occurred in each subject, but in addition, anions normally associated with intermediate metabolism were significantly elevated after exercise. The contribution of these anions to generating an acidosis, and thus potentially limiting the extent of exercise, has never been acknowledged. |
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Authors:
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William McKinnon; C Pentecost; Gwyn A Lord; Lui G Forni; Jean-Marie Peron; Philip J Hilton |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Biomedical chromatography : BMC Volume: 22 ISSN: 0269-3879 ISO Abbreviation: Biomed. Chromatogr. Publication Date: 2008 Mar |
Date Detail:
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Created Date: 2008-03-11 Completed Date: 2008-06-27 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 8610241 Medline TA: Biomed Chromatogr Country: England |
Other Details:
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Languages: eng Pagination: 301-5 Citation Subset: IM |
Affiliation:
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St Thomas's Hospital, London SE1 7EH, UK. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acidosis
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metabolism* Anions* Chromatography, Ion Exchange / methods* Exercise* Humans Male Spectrometry, Mass, Electrospray Ionization / methods* Stress, Physiological / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Anions |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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