Document Detail


Elevated procaspase levels in human melanoma.
MedLine Citation:
PMID:  11479427     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In this study procaspase expression levels were investigated by Western blotting in a panel of established melanoma cell lines, transformed melanocytic cell lines and normal primary melanocytes. Upstream caspases such as procaspase-8 that contain a death effector domain were found to be overexpressed in transformed melanocytes and melanoma cell lines compared with melanocytes. Heterogeneous levels of procaspase-8 were seen in melanoma cells, including one cell line that completely lacked procaspase-8 expression. Procaspase-10 is generally overexpressed in transformed melanocytes and melanoma cell lines. Expression of the downstream procaspases-3 and -7 was increased in melanoma cells compared with normal melanocytes. Procaspases containing caspase recruitment domains such as procaspase-2 were expressed at similar levels in nearly all the cell lines investigated. Reduced levels of procaspase-1 compared with normal melanocytes were detected in transformed melanocytes and melanoma cell lines. These data indicate that procaspase levels in general increase during the malignant transformation of melanocytic cells.
Authors:
D Fink; H Schlagbauer-Wadl; E Selzer; T Lucas; K Wolff; H Pehamberger; H G Eichler; B Jansen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Melanoma research     Volume:  11     ISSN:  0960-8931     ISO Abbreviation:  Melanoma Res.     Publication Date:  2001 Aug 
Date Detail:
Created Date:  2001-07-31     Completed Date:  2001-10-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9109623     Medline TA:  Melanoma Res     Country:  England    
Other Details:
Languages:  eng     Pagination:  385-93     Citation Subset:  IM    
Affiliation:
Department of Clinical Pharmacology, Section of Experimental Oncology/Molecular Pharmacology, University of Vienna, Waehringer Guertel 18-20, A-1090 Vienna, Austria.
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MeSH Terms
Descriptor/Qualifier:
Blotting, Western
Caspases / chemistry,  metabolism*
Cell Line, Transformed
Cell Transformation, Neoplastic / metabolism
Cells, Cultured
Enzyme Induction
Enzyme Precursors / chemistry,  metabolism*
Gene Expression Regulation, Neoplastic*
Humans
Melanocytes / cytology,  enzymology
Melanoma / enzymology*,  pathology
Molecular Weight
Tumor Cells, Cultured
Chemical
Reg. No./Substance:
0/Enzyme Precursors; EC 3.4.22.-/Caspases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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