Document Detail


Elevated levels of human endogenous retrovirus-W transcripts in blood cells from patients with first episode schizophrenia.
MedLine Citation:
PMID:  17559415     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We previously reported on the differential presence of transcripts related to the human endogenous retrovirus (HERV)-W family in cerebrospinal fluid and plasma from patients with first-episode schizophrenia compared with control individuals. Whether this is a consequence of qualitative or quantitative differences in transcription of genomic regions harboring HERV-W elements is not known. The purpose of the present study was therefore to characterize the transcribed HERV-W elements in mononuclear cells obtained from 30 patients first hospitalized for schizophrenia-related psychosis and from 26 healthy control individuals. We observed elevated total levels of HERV-W gag (2.1-fold, P < 0.01) but not env transcripts in the cells of patients compared with controls. By using the melting temperatures of the amplicons as a proxy marker for sequence identity, no absolute qualitative differences was detected between the two groups. Mapping of the detected transcripts identified several intronic and intergenic HERV-W elements transcribed in the cells, including elements previously considered transcriptionally silent. Element-specific assays revealed elevated levels of intronic transcripts containing HERV-W gag sequence from the putative gene PTD015 on chromosome 11q13.5 (1.6-fold, P < 0.05) in the patients compared with the controls. Thus, studies aiming to further understanding of complex human disease such as schizophrenia may need to be extended beyond the strictly protein-coding fraction of the transcriptome.
Authors:
Y Yao; J Schröder; C Nellåker; C Bottmer; S Bachmann; R H Yolken; H Karlsson
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-06-07
Journal Detail:
Title:  Genes, brain, and behavior     Volume:  7     ISSN:  1601-183X     ISO Abbreviation:  Genes Brain Behav.     Publication Date:  2008 Feb 
Date Detail:
Created Date:  2008-01-21     Completed Date:  2008-04-22     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101129617     Medline TA:  Genes Brain Behav     Country:  England    
Other Details:
Languages:  eng     Pagination:  103-12     Citation Subset:  IM    
Affiliation:
Department of Neuroscience, Karolinska Institutet, Stockholm, Sweden.
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MeSH Terms
Descriptor/Qualifier:
Chromosomes, Human, Pair 11
Endogenous Retroviruses / genetics*
Gene Expression Profiling
Gene Expression Regulation, Viral / physiology
Gene Products, gag / genetics
Humans
Introns / genetics
Lymphocytes / virology
Psychotic Disorders / genetics,  virology*
Reverse Transcriptase Polymerase Chain Reaction
Schizophrenia / genetics,  virology*
Sequence Analysis, DNA
Transcription, Genetic / genetics*
Viral Envelope Proteins / genetics
Chemical
Reg. No./Substance:
0/Gene Products, gag; 0/Viral Envelope Proteins

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