| Elevated levels of NO are localized to distal airways in asthma. | |
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MedLine Citation:
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PMID: 21419218 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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The contribution of nitric oxide (NO) to the pathophysiology of asthma remains incompletely defined despite its established pro- and anti-inflammatory effects. Induction of the inducible nitric oxide synthase (iNOS), arginase and superoxide pathways is correlated with increased airway hyperresponsiveness (AHR) in asthmatic subjects. To determine the contributions of these pathways in proximal and distal airways, we compared bronchial wash (BW) to traditional bronchoalveolar lavage (BAL) for measurements of reactive nitrogen/oxygen species, arginase activation, and cytokine/chemokine levels in asthmatic and normal subjects. Levels of NO were preferentially elevated in the BAL, demonstrating higher-level NOS activation in the distal airway compartment of asthmatic subjects. In contrast, DHE(+) cells which have the potential to generate reactive oxygen species were found to be increased in both proximal and distal airway compartments of asthmatics compared to controls. Different patterns of cytokines and chemokines were observed, with a predominance of epithelial cell-associated mediators in the BW as compared to macrophage/monocyte-derived mediators in the BAL of asthmatic subjects. Our study demonstrates differential production of reactive species and soluble mediators within the distal airways as compared to the proximal airways in asthma. These results indicate that cellular mechanisms are activated in the distal airways of asthmatics and must be considered in the development of therapeutic strategies for this chronic inflammatory disorder. |
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Authors:
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John T Anderson; Meiqin Zeng; Qian Li; Ryan Stapley; Doyle Ray Moore; Balachandra Chenna; Naomi Fineberg; Jaroslaw Zmijewski; Isam-Eldin Eltoum; Gene P Siegal; Amit Gaggar; Stephen Barnes; Sadanandan E Velu; Victor J Thannickal; Edward Abraham; Rakesh P Patel; Jack R Lancaster; David D Chaplin; Mark T Dransfield; Jessy S Deshane |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-3-15 |
Journal Detail:
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Title: Free radical biology & medicine Volume: - ISSN: 1873-4596 ISO Abbreviation: - Publication Date: 2011 Mar |
Date Detail:
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Created Date: 2011-3-22 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8709159 Medline TA: Free Radic Biol Med Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2010. Published by Elsevier Inc. |
Affiliation:
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Department of Medicine, Birmingham, AL. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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