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Elevated interleukin-32 expression in granulomatosis with polyangiitis.
MedLine Citation:
PMID:  22850185     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Objective. To evaluate the role of IL-32 in granulomatosis with polyangiitis (GPA) patients and the relationship between IL-32 and disease activity, as PR3 has the ability to bind and activate IL-32, which has been described as a novel cytokine that induces inflammatory cytokines.Methods. We investigated the level of IL-32, PR3, TNF-α and IL-6 in GPA patients by using ELISA. Northern blot was used to analyse the level of IL-32 mRNA in leucocytes of GPA patients. The intracellular colocalization of IL-32 and PR3 in leucocytes was examined by IF staining.Results. We observed that IL-32 and PR3 levels in GPA patients were increased significantly when compared with normal individuals and each was tightly associated (P < 0.001). Northern blot analysis revealed that the mRNA level of IL-32 was prominently elevated in leucocytes of GPA patients. The intracellular colocalization of IL-32 and PR3 in leucocytes from GPA patients vs normal individuals was verified by IF staining.Conclusion. IL-32 level was elevated in GPA patients but its level was changed by treatment response. IL-32 could be an index in GPA and play a role in the aetiology of GPA.
Authors:
Suyoung Bae; Yong-Gil Kim; Jida Choi; Jaewoo Hong; Siyoung Lee; Taebong Kang; Hyunjhung Jeon; Kwangwon Hong; Eusom Kim; Areum Kwak; Chang-Keun Lee; Bin Yoo; Yong-Beom Park; Eun Young Song; Soohyun Kim
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-7-31
Journal Detail:
Title:  Rheumatology (Oxford, England)     Volume:  -     ISSN:  1462-0332     ISO Abbreviation:  Rheumatology (Oxford)     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-8-1     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100883501     Medline TA:  Rheumatology (Oxford)     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Department Biomedical Science and Technology, Laboratory of Cytokine Immunology, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Division of Rheumatology, Department of Internal Medicine, University of Ulsan College of Medicine, Asan Medical Center, Songpa-gu, Division of Rheumatology, Department of Internal Medicine, Yonsei University, College of Medicine, Severance Hospital, Seodaemun-gu, and Department of Laboratory Medicine, Seoul National University College of Medicine, 28 Yeongeon-dong, Jongno-gu, Seoul, Korea.
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