Document Detail

Elevated expression of proto-oncogenes accompany enhanced induction of heat-shock genes after exposure of rat embryos in utero to ionizing irradiation.
MedLine Citation:
PMID:  2572063     Owner:  NLM     Status:  MEDLINE    
We have recently found that the effects of exposing rat embryos in utero to teratogens capable of producing cardiac anomalies were expressed later as enhanced induction of heat-shock proteins (hsp70 family) when embryonic hearts were cultured in vitro. However, it remained to be determined whether heat-shock proteins are induced in vivo after exposure to teratogens. The heat-shock response in some mammalian systems is known to be accompanied by elevated expression of proto-oncogenes. Using gene-specific DNA probes, we examined the levels of the expression (transcription) of heat-shock protein genes and two nuclear proto-oncogenes, c-fos and c-myc, in the embryos removed from irradiated pregnant mother rats 4 or 5 days after the irradiation. We found that the levels of expression in vivo of the hsp70 and c-myc genes in the irradiated embryos increased by approximately twofold as compared with those in the control. The expression in vivo of the c-fos gene was not detected in either the irradiated or non-irradiated embryos. After 0.5-hr incubation in vitro of the embryos, however, the expression of the c-fos gene in the irradiated embryos was highly enhanced whereas the control showed no changes. Although the exact functions of these gene products still remain obscure, the enhanced expression of hsp70 gene(s) and the nuclear proto-oncogenes observed in the present study may reflect repair of intracellular damages and/or regeneration of tissue by compensatory cell proliferation, processes that may disturb the normal program of organogenesis.
H Higo; J Y Lee; Y Satow; K Higo
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Teratogenesis, carcinogenesis, and mutagenesis     Volume:  9     ISSN:  0270-3211     ISO Abbreviation:  Teratog., Carcinog. Mutagen.     Publication Date:  1989  
Date Detail:
Created Date:  1989-11-20     Completed Date:  1989-11-20     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  8100917     Medline TA:  Teratog Carcinog Mutagen     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  191-8     Citation Subset:  IM    
Department of Geneticopathology, Hiroshima University, Japan.
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MeSH Terms
Blotting, Northern
DNA Probes
Embryo, Mammalian / radiation effects*
Gene Expression
Heat-Shock Proteins / genetics*
Multigene Family
Proto-Oncogene Proteins / biosynthesis,  genetics*
Proto-Oncogene Proteins c-fos
Proto-Oncogene Proteins c-myc
RNA, Messenger / biosynthesis,  metabolism
Reg. No./Substance:
0/DNA Probes; 0/Heat-Shock Proteins; 0/Proto-Oncogene Proteins; 0/Proto-Oncogene Proteins c-fos; 0/Proto-Oncogene Proteins c-myc; 0/RNA, Messenger

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