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Elevated cyclin D1 expression is governed by plasma IGF-1 through Ras/Raf/MEK/ERK pathway in rumen epithelium of goats supplying a high metabolizable energy diet.
MedLine Citation:
PMID:  23278864     Owner:  NLM     Status:  Publisher    
The objective of this study was to explore the underlying mechanism of insulin-like growth factor 1 (IGF-1)-caused cell proliferation of rumen epithelium in goats fed a high metabolizable energy (ME) diet. In this study, young goats were fed either a low ME [LL, n = 9, ME: 0.57 MJ/kg(0.75) /day] or high ME [HL, n = 9, ME: 1.00 MJ/(kg(0.75) /day)] diet for 42 day. The time duration of G(1) -phase was shortened as a result of enhanced expression of cyclin D1 mRNA in the HL group (p < 0.05). It was suggested that a high ME diet promoted cell transition from G(0) /G(1) to S-phase via cyclin D1. The level of phosphorylation of ERK was higher in HL than LL group (p < 0.05). In cell culture, the ERK was phosphorylated by IGF-1 treatment. The proliferative effects of insulin-like growth factor 1 (IGF-1, 25 ng/ml) on [(3) H] thymidine (TdR) incorporation into DNA and on cyclin D1 protein expression of rumen epithelial cells were inhibited by PPP (the inhibitor of type 1 IGF receptor) (p < 0.05) and ERK inhibitor (p < 0.05) in vitro. Thus, IGF-1 up-regulated cyclin D1 expression and accelerated G(1) -phase progression in the cell cycle through Ras/Raf/MEK/ERK pathway in rumen epithelium of goats.
J Lu; H Zhao; J Xu; L Zhang; L Yan; Z Shen
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-12-31
Journal Detail:
Title:  Journal of animal physiology and animal nutrition     Volume:  -     ISSN:  1439-0396     ISO Abbreviation:  J Anim Physiol Anim Nutr (Berl)     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2013-1-2     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101126979     Medline TA:  J Anim Physiol Anim Nutr (Berl)     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Journal of Animal Physiology and Animal Nutrition © 2013 Blackwell Verlag GmbH.
Lab of Animal Physiology and Biochemistry, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China; Jiangsu Animal Husbandry & Veterinary College, Nanjing, China.
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