| Elevated cholesterol and decreased sterol carrier protein-2 in peroxisomes from AS-30D hepatoma compared to normal rat liver. | |
| | |
MedLine Citation:
|
PMID: 1897930 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Peroxisomes were isolated from AS-30D hepatoma and compared to normal rat liver cells for the purpose of investigating the cholesterol accumulation in the hepatoma cells. Cholesterol was found to be approximately 10-fold higher relative to protein in AS-30D peroxisomes as compared to peroxisomes from normal liver. The peroxisomes from the hepatoma cells were found to be more stable; catalase was not released from these peroxisomes during isolation or osmotic shock of the peroxisomal fraction. The elevated cholesterol level may stabilize the peroxisomal membrane. Sterol carrier protein-2 (SCP-2) levels were measured using a radioimmunoassay (RIA), which indicated the highest concentration of SCP-2 to be in peroxisomes. Hepatoma peroxisomes had a lower concentration of SCP-2 (2.5 micrograms/mg) than normal liver peroxisomes (8 micrograms/mg). Approximately half of all SCP-2 detected was found to be soluble in both hepatoma and normal rat liver cells. Immunoblots from both rat liver and AS-30D fractions demonstrated the presence of the 14-kDa form of SCP-2. The liver fractions also had a 57-kDa immunoreactive protein, which was barely detectable in the AS-30D fractions. The low abundance of the high molecular weight form of SCP-2 from hepatoma peroxisomes and the lower amounts of SCP-2 detected in the AS-30D peroxisomes may be related to the accumulation of cholesterol in the cells. |
| | |
Authors:
|
H T Lyons; A Kharroubi; N Wolins; S Tenner; R F Chanderbhan; G Fiskum; R P Donaldson |
Publication Detail:
|
Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: Archives of biochemistry and biophysics Volume: 285 ISSN: 0003-9861 ISO Abbreviation: Arch. Biochem. Biophys. Publication Date: 1991 Mar |
Date Detail:
|
Created Date: 1991-10-23 Completed Date: 1991-10-23 Revised Date: 2007-11-14 |
Medline Journal Info:
|
Nlm Unique ID: 0372430 Medline TA: Arch Biochem Biophys Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 238-45 Citation Subset: IM |
Affiliation:
|
Department of Biological Sciences, George Washington University, Washington, D.C. 20052. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Carrier Proteins / metabolism* Cell Fractionation Centrifugation, Density Gradient Cholesterol / metabolism* Female Immunoblotting Liver / ultrastructure* Liver Neoplasms, Experimental / ultrastructure* Microbodies / enzymology, metabolism* Plant Proteins* Radioimmunoassay Rats Rats, Inbred Strains Tumor Cells, Cultured |
| Grant Support | |
ID/Acronym/Agency:
|
CA32946/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Carrier Proteins; 0/Plant Proteins; 0/sterol carrier proteins; 57-88-5/Cholesterol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Isolation of a full-length cDNA encoding mouse aromatase P450.
Next Document: A comparison of phospholipid degradation by oxidation and hydrolysis during the mitochondrial permea...