Document Detail


Elevated NADPH-oxidase activity in neutrophils from bile-duct-ligated rats: changes in the kinetic parameters and in the oxidase cytosolic factor p47.
MedLine Citation:
PMID:  8305498     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Stimulated superoxide generation was 2-fold higher in neutrophils from 20 rats with common bile-duct ligation (CBDL) compared to that of 20 sham-operated control rats. In order to study the mechanism of the higher NADPH oxidase activity in CBDL rats, the kinetic parameters of NADPH oxidase were analyzed. The Vmax of the NADPH oxidase in CBDL rat neutrophils was significantly higher than that of control rat neutrophils (10.2 and 5.3 nmol/min, respectively). The membrane and cytosol fractions of the oxidase were studied in a cell-free system. Neutrophil cytosol from CBDL rats added to neutrophil membranes from either CBDL or control rats produced 22.4 +/- 1.6 and 21.0 +/- 1.4 nmol/10(6) cells per 10 min, respectively. When neutrophil cytosol from control rats was mixed with neutrophil membranes from control or CBDL rats the generation of superoxide was 10.6 +/- 1.4 and 10.0 +/- 1.5 nmol/10(6) cells per 10 min, respectively. These results suggest that the cytosol components of the oxidase regulate its activity. By immunoblot analysis it was shown that the amount of the cytosolic factor p47 in neutrophils of CBDL rats is higher than that present in an equal number of neutrophils from control rats.
Authors:
R Levy; O Nagauker; E Sikuler; T L Leto; F Schlaeffer
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1220     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1994 Feb 
Date Detail:
Created Date:  1994-03-17     Completed Date:  1994-03-17     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  261-5     Citation Subset:  IM    
Affiliation:
Infectious Diseases Laboratory, Soroka Medical Center, Beer-Sheva, Israel.
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MeSH Terms
Descriptor/Qualifier:
Animals
Bile Ducts / physiology*
Blood Proteins / metabolism*
Cell Fractionation
Cell Membrane / metabolism
Cell-Free System
Cytosol / metabolism
Electrophoresis, Polyacrylamide Gel
Immunoblotting
Kinetics
Male
Molecular Weight
NADH, NADPH Oxidoreductases / analysis,  blood*,  isolation & purification
NADPH Oxidase
Neutrophils / enzymology*
Rats
Rats, Sprague-Dawley
Reference Values
Superoxides / blood*
Chemical
Reg. No./Substance:
0/Blood Proteins; 11062-77-4/Superoxides; EC 1.6.-/NADH, NADPH Oxidoreductases; EC 1.6.3.1/NADPH Oxidase

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