Document Detail


Electrospun nanofibrous mats containing quaternized chitosan and polylactide with in vitro antitumor activity against HeLa cells.
MedLine Citation:
PMID:  20469930     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Nanofibrous materials containing the antitumor drug doxorubicin hydrochloride (DOX) were easily prepared using a one-step method by electrospinning of DOX/poly(L-lactide-co-D,L-lactide) (coPLA) and DOX/quaternized chitosan (QCh)/coPLA solutions. The pristine and DOX-containing mats were characterized by ATR-FTIR and X-ray photoelectron spectroscopy (XPS). The release rate of DOX from the prepared fibers increased with the increase in DOX content. The DOX release process was diffusion-controlled. MTT cell viability studies revealed that incorporation of DOX and QCh in the nanofibrous mats led to a significant reduction in the HeLa cells viability. It was found, that the antitumor efficacy of the DOX-containing mats at 6 h was higher than that of the free DOX. SEM, TEM, and fluorescence microscopic observations confirmed that the antitumor effect of QCh-based and DOX-containing fibrous mats was mainly due to induction of apoptosis in the HeLa cells.
Authors:
Milena G Ignatova; Nevena E Manolova; Reneta A Toshkova; Iliya B Rashkov; Elena G Gardeva; Lilia S Yossifova; Marin T Alexandrov
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biomacromolecules     Volume:  11     ISSN:  1526-4602     ISO Abbreviation:  Biomacromolecules     Publication Date:  2010 Jun 
Date Detail:
Created Date:  2010-06-14     Completed Date:  2010-11-09     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100892849     Medline TA:  Biomacromolecules     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1633-45     Citation Subset:  IM    
Affiliation:
Laboratory of Bioactive Polymers, Institute of Polymers, Bulgarian Academy of Sciences, Acad. G. Bonchev St. bl. 103A, BG-1113 Sofia, Bulgaria.
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MeSH Terms
Descriptor/Qualifier:
Antibiotics, Antineoplastic / administration & dosage,  pharmacology*
Cell Survival / drug effects
Chitosan / chemistry*
Doxorubicin / administration & dosage,  pharmacology*
Drug Carriers / chemistry*
Electrochemical Techniques
Hela Cells
Humans
Microscopy, Electron, Scanning
Molecular Structure
Nanofibers / chemistry*
Photoelectron Spectroscopy
Polyesters / chemistry*
Spectroscopy, Fourier Transform Infrared
Surface Properties
Chemical
Reg. No./Substance:
0/Antibiotics, Antineoplastic; 0/Drug Carriers; 0/N-trimethyl chitosan chloride; 0/Polyesters; 23214-92-8/Doxorubicin; 26969-66-4/poly(lactide); 9012-76-4/Chitosan

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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