Document Detail


Electrospray ionisation mass spectrometric analysis of lipid restructuring in the carp (Cyprinus carpio L.) during cold acclimation.
MedLine Citation:
PMID:  12432020     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cold acclimation of carp from 30 degrees C to 10 degrees C causes a restructuring of liver microsomal phospholipids characterised by increased proportions of monounsaturated fatty acid in phosphatidylcholine (PC) and phosphatidylethanolamine (PE). Here, we have used electrospray ionisation mass spectrometry (ESI-MS) to determine the patterns of alteration to individual molecular species compositions of PC, PE and phosphatidylinositol (PI) in response to gradually decreasing temperature. The results demonstrate that cold induces precise changes to a limited number of phospholipid species, and that these changes are distinct and different for each phospholipid class. The major change for PC was increased 16:1/22:6, but for PE the species that increased was 18:1/22:6. By contrast, the PI species that increased during cold acclimation were characterised by an sn-1 monounsaturated fatty acid in combination with arachidonoyl or eicosapentaenoyl fatty acid at the sn-2 position. Analysis of acyl distribution indicates that cold only caused the accumulation of monounsaturated fatty acids at the sn-1 and not at the sn-2 position of phospholipids. These results highlight the tight and restricted range of modifications that membranes make to their phospholipid composition in response to thermal stress.
Authors:
S Brooks; G T Clark; S M Wright; R J Trueman; A D Postle; A R Cossins; N M Maclean
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of experimental biology     Volume:  205     ISSN:  0022-0949     ISO Abbreviation:  J. Exp. Biol.     Publication Date:  2002 Dec 
Date Detail:
Created Date:  2002-11-14     Completed Date:  2004-01-09     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0243705     Medline TA:  J Exp Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  3989-97     Citation Subset:  IM    
Affiliation:
Department of Cell Sciences, School of Biological Sciences, University of Southampton, UK.
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MeSH Terms
Descriptor/Qualifier:
Animals
Carps / physiology*
Cold Temperature*
Fatty Acids / chemistry,  metabolism
Microsomes, Liver / chemistry,  metabolism
Phospholipids / chemistry,  metabolism*
Spectrometry, Mass, Electrospray Ionization
Chemical
Reg. No./Substance:
0/Fatty Acids; 0/Phospholipids

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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