Document Detail


Electrospray ionization tandem mass spectrometry of sodiated adducts of cholesteryl esters.
MedLine Citation:
PMID:  21904795     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cholesteryl esters (CE) are important lipid storage molecules. The present study demonstrates that sodiated adducts of CE molecular species form positive ions that can be detected in both survey scan mode as well as by exploiting class-specific fragmentation in MS/MS scan modes. A common neutral loss for CE is the loss of cholestane (NL 368.5), which can be used to specifically quantify tissue CE molecular species. Using this MS/MS technique, CE molecular species were quantified in mouse monocyte-derived macrophages (J774 cells) incubated with either linoleic (18:2) or arachidonic acid (20:4). These studies revealed that arachidonic acid was not only incorporated into the CE pool, but also was elongated resulting in the accumulation of 22:4 and 24:4 CE molecular species in macrophages. Additionally, this technique was used to quantify CE molecular species present in crude lipid extracts from plasma of female mice fed a Western diet, which led to an enrichment in CE molecular species containing monounsaturated fatty acids compared to female mice fed a normal chow diet. Last, NL 368.5 spectra revealed the oxidation of the aliphatic fatty acid residues of CE molecular species containing polyunsaturated fatty acids. Taken together, these studies demonstrate the utility of using sodiated adducts of CE in conjunction with direct infusion electrospray ionization tandem mass spectrometry to rapidly quantify CE molecular species in biological samples.
Authors:
John A Bowden; Fei Shao; Carolyn J Albert; John W Lally; Robert J Brown; Jesse D Procknow; Alan H Stephenson; David A Ford
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2011-09-09
Journal Detail:
Title:  Lipids     Volume:  46     ISSN:  1558-9307     ISO Abbreviation:  Lipids     Publication Date:  2011 Dec 
Date Detail:
Created Date:  2011-11-14     Completed Date:  2012-02-29     Revised Date:  2013-06-27    
Medline Journal Info:
Nlm Unique ID:  0060450     Medline TA:  Lipids     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  1169-79     Citation Subset:  IM    
Affiliation:
Department of Biochemistry and Molecular Biology, School of Medicine, and Center for Cardiovascular Research, Saint Louis University, MO 63104, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Arachidonic Acid / analysis,  metabolism
Cholestanes / analysis,  chemistry
Cholesterol Esters / analysis*
Complex Mixtures / blood*,  chemistry
Female
Ions / chemistry
Linoleic Acid / analysis,  metabolism
Lipid Metabolism*
Macrophages / chemistry*,  cytology,  metabolism
Mice
Spectrometry, Mass, Electrospray Ionization / methods*
Tandem Mass Spectrometry / methods
Triglycerides / analysis
Grant Support
ID/Acronym/Agency:
HL074214/HL/NHLBI NIH HHS; HL088073/HL/NHLBI NIH HHS; HL098907/HL/NHLBI NIH HHS; R01 HL074214/HL/NHLBI NIH HHS; R01 HL074214-06/HL/NHLBI NIH HHS; R21 HL088073/HL/NHLBI NIH HHS; R21 HL088073-02/HL/NHLBI NIH HHS; R21 HL098907/HL/NHLBI NIH HHS; R21 HL098907-02/HL/NHLBI NIH HHS; RR019232/RR/NCRR NIH HHS; S10 RR019232/RR/NCRR NIH HHS; S10 RR019232-01/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
0/Cholestanes; 0/Cholesterol Esters; 0/Complex Mixtures; 0/Ions; 0/Triglycerides; 2197-37-7/Linoleic Acid; 506-32-1/Arachidonic Acid
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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