Document Detail


Electroretinogram recordings of Drosophila.
MedLine Citation:
PMID:  21205849     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Analysis of visual physiology in Drosophila photoreceptors has been central to understanding a number of important areas of modern biology, including the G-protein-coupled receptor cycle, phosphoinositide signaling, and calcium signaling. Analysis of photoreceptor performance and synaptic transmission are areas of neurobiology that have been studied using Drosophila photoreceptors as a model system. Electrophysiological analysis of responses to light is a powerful tool for characterizing and understanding visual transduction in Drosophila photoreceptors, and electroretinograms (ERGs) have long been used as a physiological assay in the Drosophila visual system. In these recordings, a microelectrode is placed on the eye and a reference electrode is placed elsewhere on the animal (typically in the thorax). Upon light stimulation, the voltage difference between these two electrodes is measured and displayed in real time. Because recordings are performed in live animals, all photoreceptor cells are intact and therefore surrounded by fluid containing endogenous concentrations of ions. Moreover, synaptic transmission between the photoreceptor and downstream laminar neurons can be detected.
Authors:
Patrick Dolph; Amit Nair; Padinjat Raghu
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Publication Detail:
Type:  Journal Article     Date:  2011-01-01
Journal Detail:
Title:  Cold Spring Harbor protocols     Volume:  2011     ISSN:  1559-6095     ISO Abbreviation:  Cold Spring Harb Protoc     Publication Date:  2011 Jan 
Date Detail:
Created Date:  2011-01-05     Completed Date:  2011-05-18     Revised Date:  2011-06-20    
Medline Journal Info:
Nlm Unique ID:  101524530     Medline TA:  Cold Spring Harb Protoc     Country:  United States    
Other Details:
Languages:  eng     Pagination:  pdb.prot5549     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Drosophila / physiology*
Electroretinography / methods*
Entomology / methods*
Ocular Physiological Phenomena
Photoreceptor Cells, Invertebrate / physiology*
Retina / physiology

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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