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Electroporation-induced changes in normal immature rat myoblasts (H9C2).
MedLine Citation:
PMID:  22447827     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Application of a high electric field causes an electric shock to the heart. This is utilized in defibrillation to reestablish normal contraction rhythms during dangerous arrhythmias or in cardiac arrest. If shock-induced transmembrane potentials are large enough, they can cause tissue destruction due to irreversible electroporation (EP). Also electrochemotherapy of nearby tissues may have an adverse effect on the heart. Herein, we present experimental data on effects of electroporation in culture of cardiac cells (H9C2). The electric field was applied in short pulses of 25-3250 V/cm, 50 µs each. The viability of cells was tested by MTT assay after 24 hours. For detection of DNA fragmentation, associated with apoptosis, alkaline and neutral comet assays were performed after EP. Additionally phase contrast images of cells obtained directly after EP were analyzed. Although cell images indicated disruption of cell membranes after EP with high intensities, only a few percent of apoptotic cells and no necrotic effects in the cell nucleus could be observed in comet assay tests performed 2 hours post EP. MTT viability test showed that pulse intensities above 375 V/cm are destructive for myocytes viability.
Authors:
Iwona Kaminska; Malgorzata Kotulska; Anna Stecka; Jolanta Saczko; Malgorzata Drag-Zalesinska; Teresa Wysocka; Anna Choromanska; Nina Skolucka; Rafał Nowicki; Jakub Marczak; Julita Kulbacka
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  General physiology and biophysics     Volume:  31     ISSN:  0231-5882     ISO Abbreviation:  Gen. Physiol. Biophys.     Publication Date:  2012 Mar 
Date Detail:
Created Date:  2012-03-26     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8400604     Medline TA:  Gen Physiol Biophys     Country:  Slovakia    
Other Details:
Languages:  eng     Pagination:  19-25     Citation Subset:  IM    
Affiliation:
Department of Medical Biochemistry, Wroclaw Medical University, Chalubinskiego 10, 50-367 Wroclaw, Poland. julita.kulbacka@am.wroc.pl.
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