| Electroporation enhances c-myc antisense oligodeoxynucleotide efficacy. | |
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MedLine Citation:
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PMID: 8346033 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Obtaining high transfection efficiencies and achieving appropriate intracellular concentrations and localization are two of the most important barriers to the implementation of gene targeted therapy. The efficiency of endogenous uptake of oligodeoxynucleotides (ODNs) varies from cell type to cell type and may be a limiting factor of antisense efficacy. The use of electroporation to obtain high intracellular concentrations of a synthetic ODN in essentially 100% of viable cells is described. It is also shown that the transfected ODNs initially localize to the nucleus and remain there for at least 48 hours. The cellular trafficking of electroporated ODNs is shown to be an energy dependent process. Targeting of the c-myc proto-oncogene of U937 cells by electroporation of phosphorothioate-modified ODNs results in rapid and specific suppression of this gene at ODN concentrations much lower than would otherwise be required. This technique appears to be applicable to a variety of cell types and may represent a powerful new investigate tool as well as a promising approach to the ex vivo treatment of hematologic disorders. |
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Authors:
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R Bergan; Y Connell; B Fahmy; L Neckers |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Nucleic acids research Volume: 21 ISSN: 0305-1048 ISO Abbreviation: Nucleic Acids Res. Publication Date: 1993 Jul |
Date Detail:
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Created Date: 1993-09-07 Completed Date: 1993-09-07 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0411011 Medline TA: Nucleic Acids Res Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 3567-73 Citation Subset: IM |
Affiliation:
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Clinical Pharmacology Branch, NCI, NIH, Bethesda, MD 20892. |
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Base Sequence Blotting, Western Cell Division Cell Line Cell Survival Electricity* Flow Cytometry Fluorescein-5-isothiocyanate Fluorescent Dyes Genes, myc* Mice Molecular Sequence Data Oligonucleotides, Antisense / metabolism* Phosphates Proto-Oncogene Proteins c-myc / biosynthesis, genetics* Transfection |
| Chemical | |
Reg. No./Substance:
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0/Fluorescent Dyes; 0/Oligonucleotides, Antisense; 0/Phosphates; 0/Proto-Oncogene Proteins c-myc; 13598-51-1/thiophosphoric acid; 3326-32-7/Fluorescein-5-isothiocyanate |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
| Full Text | |
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Journal Information Journal ID (nlm-ta): Nucleic Acids Res ISSN: 0305-1048 ISSN: 1362-4962 |
Article Information Download PDF  Print publication date: Day: 25 Month: 7 Year: 1993 Volume: 21 Issue: 15 First Page: 3567 Last Page: 3573 ID: 331460 PubMed Id: 8346033 |
| Electroporation enhances c-myc antisense oligodeoxynucleotide efficacy. | |
| R Bergan | |
| Y Connell | |
| B Fahmy | |
| L Neckers | |
| Clinical Pharmacology Branch, NCI, NIH, Bethesda, MD 20892. |
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Article Categories:
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