| Electroporation enhances the ability of lactobacilli to remove cholesterol. | |
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MedLine Citation:
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PMID: 21943733 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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The objective of the present study was to evaluate the effect of electroporation on the membrane properties of lactobacilli and their ability to remove cholesterol in vitro. The growth of lactobacilli cells treated at 7.5kV/cm for 4ms was increased by 0.89 to 1.96 log(10) cfu/mL upon fermentation at 37°C for 20h, the increase being attributed to the reversible and transient formation of pores and defragmentation of clumped cells. In addition, an increase of cholesterol assimilation as high as 127.2% was observed for most cells electroporated at a field strength of 7.5kV/cm for 3.5ms compared with a lower field strength of 2.5kV/cm. Electroporation also increased the incorporation of cholesterol into the cellular membrane, as shown by an increased cholesterol:phospholipids ratio (50.0-59.6%) upon treatment at 7.5kV/cm compared with treatment at 2.5kV/cm. Saturation of cholesterol was observed in different regions of the membrane bilayer such as upper phospholipids, apolar tail, and polar heads, as indicated by fluorescence anisotropy using 3 fluorescent probes. Electroporation could be a useful technique to increase the ability of lactobacilli to remove cholesterol for possible use as cholesterol-lowering adjuncts in the future. |
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Authors:
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H S Lye; A A Karim; G Rusul; M T Liong |
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Publication Detail:
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Type: - |
Journal Detail:
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Title: Journal of dairy science Volume: 94 ISSN: 1525-3198 ISO Abbreviation: - Publication Date: 2011 Oct |
Date Detail:
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Created Date: 2011-9-27 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 2985126R Medline TA: J Dairy Sci Country: - |
Other Details:
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Languages: ENG Pagination: 4820-4830 Citation Subset: - |
Copyright Information:
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Copyright © 2011 American Dairy Science Association. Published by Elsevier Inc. All rights reserved. |
Affiliation:
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School of Industrial Technology, Universiti Sains Malaysia, 11800 USM Penang, Malaysia. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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