Document Detail


Electroporation dependence on cell size: an optical tweezers study.
MedLine Citation:
PMID:  21473595     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Electropermeabilization or electroporation is the electrical disruption of a cell's membrane to introduce drugs, DNA/RNA, proteins or other therapies into the cell. Despite four decades of study, the fundamental science of the process remains poorly understood and controversial. We measured the minimum applied electric field required for permeabilization of suspended spherical cells as a function of cell radius for three cell lines. Key to this work is our using optical tweezers to precisely position individual cells and enable well-defined, repeatable measurements on cells in suspension. Our findings call into question fundamental assumptions common to all theoretical treatments that we know of. It is generally expected that for individual cells from a particular cell line, large cells should be easier to electroporate than small ones: the minimum electric field to cause electropermeabilization should scale inversely with cell diameter. We found instead, that each cell line has its own characteristic field that will, on average, cause permeabilization in cells of that line. Electropermeabilization is a stochastic process: two cells which appear identical may have different permeabilization thresholds. However for all three cell lines, we found that the minimum permeabilization field for any given cell does not depend on its size.
Authors:
Brian E Henslee; Andrew Morss; Xin Hu; Gregory Lafyatis; L James Lee
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-4-7
Journal Detail:
Title:  Analytical chemistry     Volume:  -     ISSN:  1520-6882     ISO Abbreviation:  -     Publication Date:  2011 Apr 
Date Detail:
Created Date:  2011-4-8     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370536     Medline TA:  Anal Chem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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