| Electroporation and alternating current cause membrane permeation of photodynamic cytotoxins yielding necrosis and apoptosis of cancer cells. | |
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MedLine Citation:
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PMID: 20494629 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In order to increase the permeability of cell membranes for low doses of cytostatic drugs, two bioelectrochemical methods have been compared: (a) electric pore formation in the plasma membranes by single electric impulses (electroporation), and (b) reordering of membrane structure by alternating currents (capacitively coupled). These treatments were applied to human leukemic K-562 cells and human lymphoma U-937 cells, yielding apoptotic and necrotic effects, determined by flow cytometry. Additional cell death occurs after exposure to light irradiation at wavelengths lambda > 600 nm, of cells which were electroporated and had incorporated actinomycin-C or daunomycin (daunorubicin). It is observed that drug uptake after an exponentially decaying electroporation pulse of the initial field strength Eo=1.4 kV/cm and pulse time constants in the time range 0.5-3 ms is faster than during PEMF-treatment, i.e., application of an alternating current of 16 kHz, voltage U<100 V, I=55 mA, and exposure time 20 min. However, at the low a.c. voltage of this treatment, more apoptotic and necrotic cells are produced as compared to the electroporation treatment with one exponentially decaying voltage pulse. Thus, additional photodynamic action appears to be more effective than solely drugs and electroporation as applied in clinical electrochemotherapy, and more effective than the noninvasive pulsed electromagnetic fields (PEMFs), for cancer cells in general and animals bearing tumors in particular. |
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Authors:
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Nelly Traitcheva; Hermann Berg |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't Date: 2010-03-10 |
Journal Detail:
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Title: Bioelectrochemistry (Amsterdam, Netherlands) Volume: 79 ISSN: 1878-562X ISO Abbreviation: Bioelectrochemistry Publication Date: 2010 Oct |
Date Detail:
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Created Date: 2010-08-03 Completed Date: 2010-12-17 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100953583 Medline TA: Bioelectrochemistry Country: Netherlands |
Other Details:
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Languages: eng Pagination: 257-60 Citation Subset: IM |
Copyright Information:
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2010. Published by Elsevier B.V. |
Affiliation:
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Institute of Plant Physiology "M. Popov," Bulgarian Acad. of Sciences, Sofia, Bulgaria. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Apoptosis / physiology* Cell Death / drug effects, radiation effects Cell Line, Tumor Cell Membrane Permeability / drug effects, physiology, radiation effects* Cytotoxins / metabolism* Dactinomycin / analogs & derivatives, pharmacology, therapeutic use Daunorubicin / pharmacology, therapeutic use Electrochemotherapy Electromagnetic Fields* Electroporation* Humans Leukemia, Erythroblastic, Acute / pathology Lymphoma / pathology Necrosis / metabolism* Neoplasms / pathology* Photochemotherapy* Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Cytotoxins; 20830-81-3/Daunorubicin; 50-76-0/Dactinomycin; 8052-16-2/cactinomycin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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