Document Detail

Electropolymerization of pyrrole and immobilization of glucose oxidase in a flow system: influence of the operating conditions on analytical performance.
MedLine Citation:
PMID:  9642772     Owner:  NLM     Status:  MEDLINE    
The in situ potentiostatic electropolymerization of pyrrole (Py) on a Pt electrode in a thin-layer amperometric cell and the entrapment of the enzyme glucose oxidase (GOx) for the determination of glucose are reported. Polypyrrole (PPy) is directly formed by continuous passage of a buffered solution of the monomer (0.4 M) and enzyme (250 U mL-1) at pH 7 at a flow rate of 0.05-0.1 mL min-1 under a constant applied potential of +0.85 V vs Ag/AgCl decreases. The electrosynthesis of PPy by injection of 500 microL of a Py + GOx solution in a carrier electrolyte consisting of 0.05 M phosphate buffer and 0.1 M KCl at pH 7.0 was also assayed. The influence of the electropolymerization conditions on the analytical response of the sensor to glucose was investigated. The analytical performance of the PPy/GOx sensor was also studied in terms of durability and storage life, as well as selectivity against electroactive species such as ascorbic acid and uric acid as a function of the thickness of the polymer film formed.
J C Vidal; E Garcia; J R Castillo
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biosensors & bioelectronics     Volume:  13     ISSN:  0956-5663     ISO Abbreviation:  Biosens Bioelectron     Publication Date:  1998 Mar 
Date Detail:
Created Date:  1998-08-05     Completed Date:  1998-08-05     Revised Date:  2009-07-14    
Medline Journal Info:
Nlm Unique ID:  9001289     Medline TA:  Biosens Bioelectron     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  371-82     Citation Subset:  IM    
Department of Analytical Chemistry, GEAS, Faculty of Sciences, University of Zaragoza, Spain.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Flow Injection Analysis*
Glucose / analysis
Glucose Oxidase*
Pyrroles / chemistry*
Sensitivity and Specificity
Reg. No./Substance:
0/Polymers; 0/Pyrroles; 50-99-7/Glucose; EC Oxidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  An approach to conductometric immunosensor based on phthalocyanine thin film.
Next Document:  Covalent coupling of immunoglobulin G to a poly(vinyl)alcohol-poly(acrylic acid) graft polymer as a ...