Document Detail

Electropolymerization on microelectrodes: functionalization technique for selective protein and DNA conjugation.
MedLine Citation:
PMID:  16970307     Owner:  NLM     Status:  MEDLINE    
A critical shortcoming of current surface functionalization schemes is their inability to selectively coat patterned substrates at micrometer and nanometer scales. This limitation prevents localized deposition of macromolecules at high densities, thereby restricting the versatility of the surface. A new approach for functionalizing lithographically patterned substrates that eliminates the need for alignment and, thus, is scalable to any dimension is reported. We show, for the first time, that electropolymerization of derivatized phenols can functionalize patterned surfaces with amine, aldehyde, and carboxylic acid groups and demonstrate that these derivatized groups can covalently bind molecular targets, including proteins and DNA. With this approach, electrically conducting and semiconducting materials in any lithographically realizable geometry can be selectively functionalized, allowing for the sequential deposition of a myriad of chemical or biochemical species of interest at high density to a surface with minimal cross-contamination.
Eric Stern; Steven Jay; James Bertram; Benjamin Boese; Ilona Kretzschmar; Daniel Turner-Evans; Carl Dietz; David A LaVan; Tadeusz Malinski; Tarek Fahmy; Mark A Reed
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Analytical chemistry     Volume:  78     ISSN:  0003-2700     ISO Abbreviation:  Anal. Chem.     Publication Date:  2006 Sep 
Date Detail:
Created Date:  2006-09-14     Completed Date:  2007-04-26     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370536     Medline TA:  Anal Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  6340-6     Citation Subset:  IM    
Department of Biomedical Engineering, Yale University, PO Box 208248, New Haven, Connecticut 06520, USA.
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MeSH Terms
Benzaldehydes / chemistry
DNA / chemistry*
Flavonoids / chemical synthesis*,  chemistry
Oligonucleotides / chemistry*
Phenols / chemical synthesis*,  chemistry
Phenylacetates / chemistry
Proteins / chemistry*
Serum Albumin, Bovine / chemistry
Tin Compounds / chemistry
Tyramine / chemistry
Reg. No./Substance:
0/Benzaldehydes; 0/Flavonoids; 0/Oligonucleotides; 0/Phenols; 0/Phenylacetates; 0/Proteins; 0/Serum Albumin, Bovine; 0/Tin Compounds; 0/polyphenols; 123-08-0/4-hydroxybenzaldehyde; 156-38-7/4-hydroxyphenylacetic acid; 51-67-2/Tyramine; 71243-84-0/indium tin oxide; 9007-49-2/DNA

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