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Electrophysiology of human cardiac atrial and ventricular telocytes.
MedLine Citation:
PMID:  24467431     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Telocytes (TCs) with exceptionally long cellular processes of telopodes have been described in human epicardium to act as structural supporting cells in the heart. We examined myocardial chamber-specific TCs identified in atrial and ventricular fibroblast culture using immunocytochemistry and studied their electrophysiological property by whole-cell patch clamp. Atrial and ventricular TCs with extended telopodes and alternating podoms and podomers that expressed CD34, c-Kit and PDGFR-β were identified. These cells expressed large conductance Ca(2+) -activated K(+) current (BKCa ) and inwardly rectifying K(+) current (IKir ), but not transient outward K(+) current (Ito ) and ATP-sensitive potassium current (KATP ). The active channels were functionally competent with demonstrated modulatory response to H2 S and transforming growth factor (TGF)-β1 whereby H2 S significantly inhibited the stimulatory effect of TGF-β1 on current density of both BKCa and IKir . Furthermore, H2 S attenuated TGF-β1-stimulated KCa1.1/Kv1.1 (encode BKCa ) and Kir2.1 (encode IKir ) expression in TCs. Our results show that functionally competent K(+) channels are present in human atrial and ventricular TCs and their modulation may have significant implications in myocardial physiopathology.
Authors:
Jingwei Sheng; Winston Shim; Jun Lu; Sze Yun Lim; Boon Hean Ong; Tien Siang Lim; Reginald Liew; Yeow Leng Chua; Philip Wong
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of cellular and molecular medicine     Volume:  18     ISSN:  1582-4934     ISO Abbreviation:  J. Cell. Mol. Med.     Publication Date:  2014 Feb 
Date Detail:
Created Date:  2014-01-28     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101083777     Medline TA:  J Cell Mol Med     Country:  England    
Other Details:
Languages:  eng     Pagination:  355-62     Citation Subset:  IM    
Copyright Information:
© 2014 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.
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