Document Detail


Electrophysiological properties of rat lateral parabrachial neurons in vitro.
MedLine Citation:
PMID:  10070129     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Anatomical studies have demonstrated that the lateral parabrachial nucleus (LPBN) is composed of at least seven separate subnuclei distinguished by cell morphology, spatial clustering, and afferent and efferent connectivity. We hypothesized that neurons within the subnuclear clusters of the LPBN might have distinct electrophysiological properties that correlate with cellular morphology. An in vitro slice preparation was used to intracellularly record the intrinsic properties of 64 neurons located within the external lateral (EL) and central lateral (CL) subnuclei of the LPBN in adult rats. Analysis of intrinsic properties revealed that neurons in the EL subnucleus had significantly wider action potentials and on the average demonstrated more spike frequency adaptation during 2 s of depolarization compared with CL neurons. The majority of both EL and CL area neurons expressed delayed excitation (DE) after membrane hyperpolarization. DE was eliminated with the A-current blocker 4-aminopyridine (1.5-5 mM). Postinhibitory rebound was also observed in a subpopulation of EL and CL neurons. Morphological analysis of 11 LPBN neurons, which were electrophysiologically characterized and filled with 2% biocytin, failed to demonstrate an association between morphology and the electrophysiological profiles of LPBN neurons. The lack of distinct "type" of neuron within a single subnucleus of the LPBN is in agreement with recent findings reported from the neonatal rat.
Authors:
L F Hayward; R B Felder
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The American journal of physiology     Volume:  276     ISSN:  0002-9513     ISO Abbreviation:  Am. J. Physiol.     Publication Date:  1999 Mar 
Date Detail:
Created Date:  1999-04-15     Completed Date:  1999-04-15     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370511     Medline TA:  Am J Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  R696-706     Citation Subset:  IM    
Affiliation:
Department of Internal Medicine and Cardiovascular Center, University of Iowa College of Medicine and Medical Service, Department of Veterans Affairs Medical Center, Iowa City, Iowa 52242, USA.
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MeSH Terms
Descriptor/Qualifier:
Action Potentials / physiology
Animals
Electrophysiology
Lysine / analogs & derivatives
Male
Membrane Potentials / physiology
Neural Inhibition / physiology
Neurons / cytology,  physiology*
Pons / cytology,  physiology*
Rats
Rats, Sprague-Dawley
Reaction Time / physiology
Grant Support
ID/Acronym/Agency:
HL-14388/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
56-87-1/Lysine; 576-19-2/biocytin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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