Document Detail

Electrophysiological and neurochemical characterization of neurons of the medial preoptic area in Japanese quail (Coturnix japonica).
MedLine Citation:
PMID:  15542078     Owner:  NLM     Status:  MEDLINE    
Intracellular recordings of medial preoptic neurons demonstrated that most neurons show a spontaneous firing, a linear I-V relationship and low-threshold-like events suppressed by the application of Ni2+. Some neurons had a depolarizing sag of the membrane potential in response to hyperpolarizing current pulses. The majority of the cells exhibited a robust spontaneous synaptic activity suppressed by SR95531 (100 microM), a GABAA receptor antagonist, and/or by 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX, 10 microM), an (RS)-alpha-amino-3-hydroxy-5-methylisoxazole-4-propionic acid (AMPA)/kainate (KA) glutamate receptor antagonist. Most neurons were affected by the application of AMPA (10 microM), kainate (30 microM), N-methyl-D-aspartic acid (NMDA, 10 microM), isoguvacine (a GABAA receptor agonist, 100 microM), dopamine (100 microM), and norepinephrine (100 microM). Biocytin injections coupled to aromatase immunocytochemistry identified 19 recorded neurons including 3 displaying a dense aromatase immunoreactivity. All of them responded to kainate, dopamine, and norepinephrine, while only one responded to isoguvacine and NMDA. Taken together, these results demonstrate a relative electrical and neurochemical homogeneity of the medial preoptic neurons, including a few aromatase-immunoreactive neurons that could be identified by immunocytochemistry after biocytin labeling of the recorded neurons.
Charlotte A Cornil; Vincent Seutin; Patrick Motte; Jacques Balthazart
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Brain research     Volume:  1029     ISSN:  0006-8993     ISO Abbreviation:  Brain Res.     Publication Date:  2004 Dec 
Date Detail:
Created Date:  2004-11-15     Completed Date:  2005-01-24     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0045503     Medline TA:  Brain Res     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  224-40     Citation Subset:  IM    
Center for Cellular and Molecular Neurobiology, Research Group in Behavioral Neuroendocrinology, University of Liège, 17 Place Delcour (Bat L1), B-4020 Liège, Belgium.
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MeSH Terms
6-Cyano-7-nitroquinoxaline-2,3-dione / pharmacology
Aromatase / metabolism
Coturnix / physiology*
Dopamine / pharmacology
Excitatory Amino Acid Agonists / pharmacology
Excitatory Amino Acid Antagonists / pharmacology
Excitatory Postsynaptic Potentials / drug effects,  physiology*
GABA Agonists / pharmacology
GABA Antagonists / pharmacology
Isonicotinic Acids / pharmacology
Kainic Acid / pharmacology
N-Methylaspartate / pharmacology
Neurons / physiology*
Norepinephrine / pharmacology
Preoptic Area / cytology,  physiology*
Pyridazines / pharmacology
Sympathomimetics / pharmacology
alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid / pharmacology
Grant Support
Reg. No./Substance:
0/Excitatory Amino Acid Agonists; 0/Excitatory Amino Acid Antagonists; 0/GABA Agonists; 0/GABA Antagonists; 0/Isonicotinic Acids; 0/Pyridazines; 0/Sympathomimetics; 104104-50-9/gabazine; 115066-14-3/6-Cyano-7-nitroquinoxaline-2,3-dione; 487-79-6/Kainic Acid; 51-41-2/Norepinephrine; 6384-92-5/N-Methylaspartate; 64603-90-3/isoguvacine; 77521-29-0/alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid; EC

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