Document Detail


Electrophysiological and morphological features underlying neurotransmission efficacy at the splanchnic nerve-chromaffin cell synapse of bovine adrenal medulla.
MedLine Citation:
PMID:  19940070     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The ability of adrenal chromaffin cells to fast-release catecholamines relies on their capacity to fire action potentials (APs). However, little attention has been paid to the requirements needed to evoke the controlled firing of APs. Few data are available in rodents and none on the bovine chromaffin cell, a model extensively used by researchers. The aim of this work was to clarify this issue. Short puffs of acetylcholine (ACh) were fast perifused to current-clamped chromaffin cells and produced the firing of single APs. Based on the currents generated by such ACh applications and previous literature, current waveforms that efficiently elicited APs at frequencies up to 20 Hz were generated. Complex waveforms were also generated by adding simple waveforms with different delays; these waveforms aimed at modeling the stimulation patterns that a chromaffin cell would conceivably undergo upon strong synaptic stimulation. Cholinergic innervation was assessed using the acetylcholinesterase staining technique on the supposition that the innervation pattern is a determinant of the kind of stimuli chromaffin cells can receive. It is concluded that 1) a reliable method to produce frequency-controlled APs by applying defined current injection waveforms is achieved; 2) the APs thus generated have essentially the same features as those spontaneously emitted by the cell and those elicited by fast-ACh perifusion; 3) the higher frequencies attainable peak at around 30 Hz; and 4) the bovine adrenal medulla shows abundant cholinergic innervation, and chromaffin cells show strong acetylcholinesterase staining, consistent with a tight cholinergic presynaptic control of firing frequency.
Authors:
Antonio M G de Diego
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-11-25
Journal Detail:
Title:  American journal of physiology. Cell physiology     Volume:  298     ISSN:  1522-1563     ISO Abbreviation:  Am. J. Physiol., Cell Physiol.     Publication Date:  2010 Feb 
Date Detail:
Created Date:  2010-01-25     Completed Date:  2010-02-04     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100901225     Medline TA:  Am J Physiol Cell Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  C397-405     Citation Subset:  IM    
Affiliation:
Departamento de Farmacología y Terapéutica, Facultad de Medicina, Instituto Teófilo Hernando, Universidad Autónoma de Madrid, Arzobispo Morcillo, 4, 28029 Madrid, Spain. antoniomiguel.garcia@uam.es
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MeSH Terms
Descriptor/Qualifier:
Acetylcholine / metabolism*
Acetylcholinesterase / metabolism
Adrenal Medulla / innervation*
Animals
Catecholamines / metabolism*
Cattle
Cells, Cultured
Cholinergic Fibers / metabolism*
Chromaffin Cells / enzymology,  metabolism*
Electric Stimulation
Evoked Potentials
Female
Kinetics
Patch-Clamp Techniques
Presynaptic Terminals / metabolism
Splanchnic Nerves / metabolism*
Synaptic Transmission*
Chemical
Reg. No./Substance:
0/Catecholamines; 51-84-3/Acetylcholine; EC 3.1.1.7/Acetylcholinesterase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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