| Electrophysiological evidence for acidic, basic, and neutral amino acid olfactory receptor sites in the catfish. | |
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MedLine Citation:
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PMID: 6481334 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Electrophysiological experiments indicate that olfactory receptors of the channel catfish, Ictalurus punctatus, contain different receptor sites for the acidic (A), basic (B), and neutral amino acids; further, at least two partially interacting neutral sites exist, one for the hydrophilic neutral amino acids containing short side chains (SCN), and the second for the hydrophobic amino acids containing long side chains (LCN). The extent of cross-adaptation was determined by comparing the electro-olfactogram (EOG) responses to 20 "test" amino acids during continuous bathing of the olfactory mucosa with water only (control) to those during each of the eight "adapting" amino acid regimes. Both the adapting and test amino acids were adjusted in concentrations to provide approximately equal response magnitudes in the unadapted state. Under all eight adapting regimes, the test EOG responses were reduced from those obtained in the unadapted state, but substantial quantitative differences resulted, depending upon the molecular structure of the adapting stimulus. Analyses of the patterns of EOG responses to the test stimuli identified and characterized the respective "transduction processes," a term used to describe membrane events initiated by a particular subset of amino acid stimuli that are intricately linked to the origin of the olfactory receptor potential. Only when the stimulus compounds interact with different transduction processes are the stimuli assumed to bind to different membrane "sites." Four relatively independent L-alpha-amino acid transduction processes (and thus at least four binding sites) identified in this report include: (a) the A process for aspartic and glutamic acids; (b) the B process for arginine and lysine; (c) the SCN process for glycine, alanine, serine, glutamine, and possibly cysteine; (d) the LCN process for methionine, ethionine, valine, norvaline, leucine, norleucine, glutamic acid-gamma-methyl ester, histidine, phenylalanine, and also possibly cysteine. The specificities of these olfactory transduction processes in the catfish are similar to those for the biochemically determined receptor sites for amino acids in other species of fishes and to amino acid transport specificities in tissues of a variety of organisms. |
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Authors:
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J Caprio; R P Byrd |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The Journal of general physiology Volume: 84 ISSN: 0022-1295 ISO Abbreviation: J. Gen. Physiol. Publication Date: 1984 Sep |
Date Detail:
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Created Date: 1984-11-20 Completed Date: 1984-11-20 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 2985110R Medline TA: J Gen Physiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 403-22 Citation Subset: IM |
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adaptation, Physiological Amino Acids / metabolism* Animals Binding Sites Electrophysiology Fishes Hydrogen-Ion Concentration Sensory Receptor Cells / metabolism* Smell / physiology* |
| Grant Support | |
ID/Acronym/Agency:
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NS14819/NS/NINDS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
| Full Text | |
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Journal Information Journal ID (nlm-ta): J Gen Physiol ISSN: 0022-1295 ISSN: 1540-7748 Publisher: The Rockefeller University Press |
Article Information Download PDF  Print publication date: Day: 1 Month: 9 Year: 1984 Volume: 84 Issue: 3 First Page: 403 Last Page: 422 ID: 2228740 Publisher Id: 85008672 PubMed Id: 6481334 |
| Electrophysiological evidence for acidic, basic, and neutral amino acid olfactory receptor sites in the catfish | |
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