Document Detail


Electrophysiological characterization of tight junctional pathway of rabbit cornea treated with ophthalmic ingredients.
MedLine Citation:
PMID:  18057726     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The purpose of this study was to investigate the continuous and real-time influence of ophthalmic ingredients on rabbit cornea by monitoring electrophysiological characteristics. The tight junctional permeabilities of FITC-dextran 4,400 (FD-4) was also determined through the cornea in the presence of ophthalmic ingredients. Intact cornea showed approximately one k-ohmxcm(2) of transepithelial electrical resistance (TEER) and extremely low permeability of FD-4. The ophthalmic ingredients used in the present study were benzalkonium chloride (BK; 0.002%, 0.01%, 0.05%), ethylenediaminetetraacetic acid (EDTA; 0.5%), capric acid (C10; 0.25%), saponin (SP; 0.1%), taurocholic acid (TA; 1.0%) and sodium dodecyl sulfate (SDS; 0.01%). They were previously reported to be effective on corneal penetrations of various drugs at those concentrations without severe toxicity. These ingredients decreased TEER and increased corneal permeability of FD-4. BK reduced TEER in a concentration-dependent manner. There was a significant correlation (gamma=0.860) between the permeability coefficient (Papp) of FD-4 and conductance (Gm), which is the reciprocal value of TEER. It was also indicated that Papp and Gm have a relationship with the corneal cytotoxicity of the ingredients. In conclusion, an electrophysiological method using isolated cornea was very useful to determine the continuous and real-time influence of ophthalmic ingredients on the cornea. In this method, electrophysiological conductance must be able to predict corneal tight junction permeability and the corneal cytotoxicity of ingredients.
Authors:
Tadahiro Nakamura; Mikiko Yamada; Mugen Teshima; Mikiro Nakashima; Hideto To; Nobuhiro Ichikawa; Hitoshi Sasaki
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  Biological & pharmaceutical bulletin     Volume:  30     ISSN:  0918-6158     ISO Abbreviation:  Biol. Pharm. Bull.     Publication Date:  2007 Dec 
Date Detail:
Created Date:  2007-12-06     Completed Date:  2008-01-02     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9311984     Medline TA:  Biol Pharm Bull     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  2360-4     Citation Subset:  IM    
Affiliation:
Department of Hospital Pharmacy, Nagasaki University Hospital of Medicine and Dentistry, 1-7-1 Sakamoto, Nagasaki 852-8501, Japan.
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MeSH Terms
Descriptor/Qualifier:
Absorption
Animals
Cell Membrane Permeability / drug effects
Cell Survival / drug effects
Cornea / cytology*,  drug effects
Data Interpretation, Statistical
Dextrans
Diffusion Chambers, Culture
Electrophysiology
Fluorescein-5-isothiocyanate
Male
Ophthalmic Solutions / pharmacology*
Rabbits
Signal Transduction / drug effects*
Tight Junctions / drug effects*
Chemical
Reg. No./Substance:
0/Ophthalmic Solutions; 3326-32-7/Fluorescein-5-isothiocyanate; 9004-54-0/Dextrans

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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