| Electrophysiological characterization of tight junctional pathway of rabbit cornea treated with ophthalmic ingredients. | |
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MedLine Citation:
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PMID: 18057726 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The purpose of this study was to investigate the continuous and real-time influence of ophthalmic ingredients on rabbit cornea by monitoring electrophysiological characteristics. The tight junctional permeabilities of FITC-dextran 4,400 (FD-4) was also determined through the cornea in the presence of ophthalmic ingredients. Intact cornea showed approximately one k-ohmxcm(2) of transepithelial electrical resistance (TEER) and extremely low permeability of FD-4. The ophthalmic ingredients used in the present study were benzalkonium chloride (BK; 0.002%, 0.01%, 0.05%), ethylenediaminetetraacetic acid (EDTA; 0.5%), capric acid (C10; 0.25%), saponin (SP; 0.1%), taurocholic acid (TA; 1.0%) and sodium dodecyl sulfate (SDS; 0.01%). They were previously reported to be effective on corneal penetrations of various drugs at those concentrations without severe toxicity. These ingredients decreased TEER and increased corneal permeability of FD-4. BK reduced TEER in a concentration-dependent manner. There was a significant correlation (gamma=0.860) between the permeability coefficient (Papp) of FD-4 and conductance (Gm), which is the reciprocal value of TEER. It was also indicated that Papp and Gm have a relationship with the corneal cytotoxicity of the ingredients. In conclusion, an electrophysiological method using isolated cornea was very useful to determine the continuous and real-time influence of ophthalmic ingredients on the cornea. In this method, electrophysiological conductance must be able to predict corneal tight junction permeability and the corneal cytotoxicity of ingredients. |
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Authors:
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Tadahiro Nakamura; Mikiko Yamada; Mugen Teshima; Mikiro Nakashima; Hideto To; Nobuhiro Ichikawa; Hitoshi Sasaki |
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Publication Detail:
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Type: In Vitro; Journal Article |
Journal Detail:
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Title: Biological & pharmaceutical bulletin Volume: 30 ISSN: 0918-6158 ISO Abbreviation: Biol. Pharm. Bull. Publication Date: 2007 Dec |
Date Detail:
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Created Date: 2007-12-06 Completed Date: 2008-01-02 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9311984 Medline TA: Biol Pharm Bull Country: Japan |
Other Details:
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Languages: eng Pagination: 2360-4 Citation Subset: IM |
Affiliation:
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Department of Hospital Pharmacy, Nagasaki University Hospital of Medicine and Dentistry, 1-7-1 Sakamoto, Nagasaki 852-8501, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Absorption Animals Cell Membrane Permeability / drug effects Cell Survival / drug effects Cornea / cytology*, drug effects Data Interpretation, Statistical Dextrans Diffusion Chambers, Culture Electrophysiology Fluorescein-5-isothiocyanate Male Ophthalmic Solutions / pharmacology* Rabbits Signal Transduction / drug effects* Tight Junctions / drug effects* |
| Chemical | |
Reg. No./Substance:
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0/Ophthalmic Solutions; 3326-32-7/Fluorescein-5-isothiocyanate; 9004-54-0/Dextrans |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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