Document Detail


Electrophoretic transport of solutes in aqueous two-phase systems.
MedLine Citation:
PMID:  1705224     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Electrophoretic transport of proteins across the interface between the phases of an aqueous polymer two-phase system can be greatly impeded in comparison with transport within the individual phase. This effect can be controlled by modifying the affinity of the protein for a phase by suitable manipulations of such variables as pH. The effect is not caused by differences in the electrophoretic velocity between the two phases, nor by large changes in pH at the interface. An analogy exists between this phenomenon and the related subject of diffusion of electrolytes across the phase interface.
Authors:
M L Levine; M Bier
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Electrophoresis     Volume:  11     ISSN:  0173-0835     ISO Abbreviation:  Electrophoresis     Publication Date:  1990 Aug 
Date Detail:
Created Date:  1991-04-01     Completed Date:  1991-04-01     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8204476     Medline TA:  Electrophoresis     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  605-11     Citation Subset:  IM    
Affiliation:
Department of Chemical Engineering, University of Arizona, Tucson.
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MeSH Terms
Descriptor/Qualifier:
Buffers
Coloring Agents
Dextrans
Diffusion*
Electrophoresis*
Globins / chemistry*
Hemoglobins / chemistry
Hydrogen-Ion Concentration
Kinetics
Myoglobin / chemistry
Polyethylene Glycols
Serum Albumin / chemistry
Thermodynamics
Chemical
Reg. No./Substance:
0/Buffers; 0/Coloring Agents; 0/Hemoglobins; 0/Myoglobin; 0/Polyethylene Glycols; 0/Serum Albumin; 9004-22-2/Globins; 9004-54-0/Dextrans

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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