Document Detail


Electrophoretic karyotyping without the need for generating protoplasts.
MedLine Citation:
PMID:  2253276     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Chromosome samples for pulsed-field electrophoresis have been prepared without first generating protoplasts. The technique involves treatment of intact, agarose-solidified cell material with protease in the presence of EDTA and SDS. Saccharomyces cerevisiae, Ustilago hordei, Tilletia caries, and T. controversa karyotypes are clearly resolved with this technique. Colonies of U. hordei and S. cerevisiae removed from the surface of agar-solidified media and prepared for PFGE by this abbreviated method also yield well resolved karyotypes.
Authors:
K McCluskey; B W Russell; D Mills
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Current genetics     Volume:  18     ISSN:  0172-8083     ISO Abbreviation:  Curr. Genet.     Publication Date:  1990 Nov 
Date Detail:
Created Date:  1991-01-24     Completed Date:  1991-01-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8004904     Medline TA:  Curr Genet     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  385-6     Citation Subset:  IM    
Affiliation:
Department of Botany and Plant Pathology, Oregon State University, Corvallis 97331-2902.
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MeSH Terms
Descriptor/Qualifier:
Chromosomes, Fungal*
Electrophoresis, Agar Gel
Fungi / genetics*
Karyotyping / methods*
Protoplasts

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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