Document Detail

Electrophoretic karyotyping without the need for generating protoplasts.
MedLine Citation:
PMID:  2253276     Owner:  NLM     Status:  MEDLINE    
Chromosome samples for pulsed-field electrophoresis have been prepared without first generating protoplasts. The technique involves treatment of intact, agarose-solidified cell material with protease in the presence of EDTA and SDS. Saccharomyces cerevisiae, Ustilago hordei, Tilletia caries, and T. controversa karyotypes are clearly resolved with this technique. Colonies of U. hordei and S. cerevisiae removed from the surface of agar-solidified media and prepared for PFGE by this abbreviated method also yield well resolved karyotypes.
K McCluskey; B W Russell; D Mills
Related Documents :
19058556 - Chromosome study of anodonta anatina (l., 1758) (bivalvia, unionidae).
23702536 - Analysis of the trojan y-chromosome eradication strategy for an invasive species.
6201326 - Preparation of chromosome suspensions for flow cytometry.
511156 - Partial translocation of nor and its activity in a balanced carrier and in her cri-du-c...
8364186 - Disappearance of ph1 chromosome with intensive chemotherapy and detection of minimal re...
10521686 - Genotoxicity assessment of new synthesized acridine derivative--3,6-diamino-10-methyl-9...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Current genetics     Volume:  18     ISSN:  0172-8083     ISO Abbreviation:  Curr. Genet.     Publication Date:  1990 Nov 
Date Detail:
Created Date:  1991-01-24     Completed Date:  1991-01-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8004904     Medline TA:  Curr Genet     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  385-6     Citation Subset:  IM    
Department of Botany and Plant Pathology, Oregon State University, Corvallis 97331-2902.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Chromosomes, Fungal*
Electrophoresis, Agar Gel
Fungi / genetics*
Karyotyping / methods*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  The chloroplast psbK operon from mustard (Sinapis alba L.): multiple transcripts during seedling dev...
Next Document:  Attitudes of medical students toward smoking