Document Detail


An electrophoretic mobility shift assay for the identification and kinetic analysis of acetyl transferase inhibitors.
MedLine Citation:
PMID:  20338149     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Histone acetyl transferases are important regulators of cellular homeostasis. This study describes a sensitive acetyl transferase electrophoretic mobility shift assay applicable both for kinetic analysis of acetyl transferase inhibitors and for high-throughput testing. Application of the assay for human GCN5L2 enabled dissection of inhibitor competition with respect to acetyl coenzyme A. Furthermore, we demonstrated that the assay can detect time-dependent inhibition of human GCN5L2 by reactive inhibitors.
Authors:
Caroline Fanslau; Donna Pedicord; Sujatha Nagulapalli; Hillary Gray; Suhong Pang; Lata Jayaraman; Jonathan Lippy; Yuval Blat
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Publication Detail:
Type:  Journal Article     Date:  2010-03-23
Journal Detail:
Title:  Analytical biochemistry     Volume:  402     ISSN:  1096-0309     ISO Abbreviation:  Anal. Biochem.     Publication Date:  2010 Jul 
Date Detail:
Created Date:  2010-05-14     Completed Date:  2010-08-09     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370535     Medline TA:  Anal Biochem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  65-8     Citation Subset:  IM    
Copyright Information:
2010 Elsevier Inc. All rights reserved.
Affiliation:
Mechanistic Biochemistry, Bristol-Myers Squibb, Princeton, NJ 08543, USA.
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MeSH Terms
Descriptor/Qualifier:
Acetyl Coenzyme A / metabolism
Animals
Cell Line
Electrophoretic Mobility Shift Assay / methods*
Histone Acetyltransferases / antagonists & inhibitors*,  metabolism*
Humans
Kinetics
Chemical
Reg. No./Substance:
72-89-9/Acetyl Coenzyme A; EC 2.3.1.48/Histone Acetyltransferases; EC 2.3.1.48/KAT2A protein, human

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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