Document Detail

Electronic separation of biological cells by volume.
MedLine Citation:
PMID:  5891056     Owner:  NLM     Status:  MEDLINE    
A device capable of separating biological cells (suspended in a conducting medium) according to volume has been developed. Cell volume is measured in a Coulter aperture, and the cells are subsequently isolated in droplets of the medium which are charged according to the sensed volume. The charged droplets then enter an electrostatic field and are deflected into a collection vessel. Mixtures of mouse and human erythrocytes and a large volume component of mouse lymphoma cells were separated successfully. In tests with Chinese hamster ovary cells essentially all cells survived separation and grew at their normal rate.
M J Fulwyler
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  Science (New York, N.Y.)     Volume:  150     ISSN:  0036-8075     ISO Abbreviation:  Science     Publication Date:  1965 Nov 
Date Detail:
Created Date:  1965-12-29     Completed Date:  1965-12-29     Revised Date:  2011-11-30    
Medline Journal Info:
Nlm Unique ID:  0404511     Medline TA:  Science     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  910-1     Citation Subset:  IM    
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MeSH Terms
Cell Biology / instrumentation*
Culture Media
Culture Techniques / instrumentation*
Electronics / instrumentation*
Ovary / cytology
Reg. No./Substance:
0/Culture Media

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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