Document Detail

Electron spin resonance spectroscopy of serum albumin: a novel new test for cancer diagnosis and monitoring.
MedLine Citation:
PMID:  16990414     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Proteins released by tumor cells can bind to serum albumin, leading to structural and functional modifications. We used electron spin resonance (ESR) spectroscopy to measure these changes in serum albumin and evaluate their utility for the diagnosis and monitoring of cancer. METHODS: We used an ESR spectrometer and 16-doxyl stearic acid as spin probe to measure conformational changes in albumin in blood samples from a population of healthy donors and volunteers (n=349), patients with a wide variety of hematologic and nonhematologic malignancy (n=135), and patients with chronic diseases such as gastrointestinal and pulmonary disease, diabetes, and cirrhosis (n=91). We added differing amounts of 16-doxyl stearic acid spin probe in ethanol to 50 microL of serum from each patient to create 3 different aliquots that differed in concentration of spin probe and ethanol, then incubated the aliquots for 10 min at 37 degrees C with continuous shaking. We measured the ESR spectra of each aliquot in triplicate and used proprietary software (MedInnovation GmbH) to evaluate the ESR spectrum for differences between cancer patients and the other groups. RESULTS: The diagnostic sensitivity and specificity of this test were 87.4% and 95.7%, respectively, for differentiating healthy individuals from cancer patients and 87.4%, and 85.7% for differentiating cancer patients from chronic disease patients. Serial evaluation of albumin conformation changes in several patients followed during the course of their disease showed excellent agreement between the magnitude of abnormality in the ESR spectrum of albumin and clinical and pathologic estimates of disease severity. CONCLUSIONS: ESR spectroscopy of serum albumin is a sensitive and noninvasive technique that clearly demonstrates diagnostic utility in patients with cancer. This test also enables monitoring of the disease course through use of serial measurements.
Steven C Kazmierczak; Andrey Gurachevsky; Gert Matthes; Vladimir Muravsky
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Publication Detail:
Type:  Evaluation Studies; Journal Article     Date:  2006-09-21
Journal Detail:
Title:  Clinical chemistry     Volume:  52     ISSN:  0009-9147     ISO Abbreviation:  Clin. Chem.     Publication Date:  2006 Nov 
Date Detail:
Created Date:  2006-10-27     Completed Date:  2006-12-05     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9421549     Medline TA:  Clin Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2129-34     Citation Subset:  IM    
Department of Pathology, Oregon Health & Science University, Portland, OR 97239, USA, and Institute of Transfusion Medicine, University Hospital Leipzig, Germany.
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MeSH Terms
Age Distribution
Aged, 80 and over
Diagnosis, Differential
Electron Spin Resonance Spectroscopy / methods*
Middle Aged
Neoplasms / blood,  diagnosis*
Predictive Value of Tests
Reference Values
Reproducibility of Results
Sensitivity and Specificity
Serum Albumin / chemistry*
Reg. No./Substance:
0/Serum Albumin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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