Document Detail


Electron paramagnetic resonance monitoring of ischemia-induced myocardial oxygen depletion and acidosis in isolated rat hearts using soluble paramagnetic probes.
MedLine Citation:
PMID:  22162021     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A new low-field electron paramagnetic resonance approach for noninvasive measurements of myocardial oxygen tension and tissue acidity was developed. The approach was applied to monitor myocardial pO(2) and pH in a model of global no-flow ischemia (30 min) and reperfusion in isolated perfused rat hearts. The myocardial oxygen measurements were performed using deuterated Finland trityl radical probe. A rapid decrease in myocardial pO(2) from 160 mmHg to about 2 ± 1 mmHg was observed within the first minute of ischemia followed by incomplete restoration of pO(2) to 50 mmHg during 30 min of reperfusion. The lower oxygen concentration after ischemia was attributed to the 50% reduction in coronary flow after ischemia as a consequence of myocardial ischemia and reperfusion damage. Myocardial pH measurements using a specially designed imidazoline pH-sensitive nitroxide showed severe myocardial acidification to pH 6.25 during 30 min of ischemia. Preconditioning of the hearts with two 5-min periods of ischemia significantly reduced the acidification of myocardial tissue during sustained ischemia. Noninvasive electron paramagnetic resonance monitoring of myocardial oxygenation and pH may provide important insights into the mechanisms of ischemia and reperfusion injury and a background for development of new therapeutic approaches.
Authors:
Denis A Komarov; Ilirian Dhimitruka; Igor A Kirilyuk; Dmitrii G Trofimiov; Igor A Grigor'ev; Jay L Zweier; Valery V Khramtsov
Publication Detail:
Type:  In Vitro; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2011-12-12
Journal Detail:
Title:  Magnetic resonance in medicine : official journal of the Society of Magnetic Resonance in Medicine / Society of Magnetic Resonance in Medicine     Volume:  68     ISSN:  1522-2594     ISO Abbreviation:  Magn Reson Med     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-07-13     Completed Date:  2012-11-30     Revised Date:  2014-09-14    
Medline Journal Info:
Nlm Unique ID:  8505245     Medline TA:  Magn Reson Med     Country:  United States    
Other Details:
Languages:  eng     Pagination:  649-55     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 Wiley Periodicals, Inc.
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MeSH Terms
Descriptor/Qualifier:
Acidosis / diagnosis*,  etiology,  metabolism*
Animals
Electron Spin Resonance Spectroscopy
Male
Molecular Probe Techniques
Molecular Probes / chemistry*,  diagnostic use
Myocardial Ischemia / complications,  diagnosis*,  metabolism*
Oximetry / methods*
Oxygen / analysis*
Rats
Rats, Sprague-Dawley
Reproducibility of Results
Sensitivity and Specificity
Solubility
Grant Support
ID/Acronym/Agency:
HL089036/HL/NHLBI NIH HHS; HL3834/HL/NHLBI NIH HHS; R01 HL038324/HL/NHLBI NIH HHS; R01 HL038324-20/HL/NHLBI NIH HHS; R21 HL089036/HL/NHLBI NIH HHS; R21 HL089036-02/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Molecular Probes; S88TT14065/Oxygen
Comments/Corrections

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