Document Detail


Electron microscopy of silver-stained core-like structures in metaphase chromosomes.
MedLine Citation:
PMID:  6181857     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Chinese hamster metaphase chromosomes, stained with ammoniacal silver and examined by electron microscopy, were covered with fine silver grains of variable size. In addition, many chromosomes contained linear aggregates of silver grains running continuously from one end of each chromatid to the other, forming a core-like structure. Extensive deposits of silver were observed over the nucleolar organizers and the centromeric regions, and the silver precipitate in the latter region appeared to be a localized differentiation of the core-like structure. The silver cores ranged from thin to thick and continuous to discontinuous elements in different chromosomes. This extreme variability suggests that these cores are not true structural components of chromosomes. The amount of silver deposit over any given chromosome region may simply reflect the concentration of chromatin in that region of the chromosome. The silver-stained core-like structure probably reflects an underlying difference in the concentration of chromatin in the central and peripheral region of each chromatid. Such differences in chromatin concentration may be induced during the prolonged hypotonic treatment required for the subsequent visualization of cores.
Authors:
G D Burkholder; M Z Kaiserman
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Canadian journal of genetics and cytology. Journal canadien de génétique et de cytologie     Volume:  24     ISSN:  0008-4093     ISO Abbreviation:  Can. J. Genet. Cytol.     Publication Date:  1982  
Date Detail:
Created Date:  1982-12-16     Completed Date:  1982-12-16     Revised Date:  2007-07-24    
Medline Journal Info:
Nlm Unique ID:  0372706     Medline TA:  Can J Genet Cytol     Country:  CANADA    
Other Details:
Languages:  eng     Pagination:  193-9     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Chromosomes / ultrastructure*
Cricetinae
Cricetulus
Metaphase
Microscopy, Electron
Silver
Staining and Labeling
Chemical
Reg. No./Substance:
7440-22-4/Silver

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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