| Electron microscopy and immunoelectronmicroscopy of cytoplasmic hepatitis B antigen in hepatocytes. | |
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MedLine Citation:
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PMID: 4134380 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The localization of hepatitis B antigen (HB Ag) and the nature of the virus-like particles in hepatocytes of patients with HB antigenemia are controversial. In many reports, numerous virus-like particles have been demonstrated in hepatocytic nuclei; the few reported in the cytoplasm are insufficient in number to explain the intense cytoplasmic fluorescence after staining with fluoresceinated antibody to HB Ag (HB Ab). We found numerous tubular and circular structures, measuring 20 to 30 nm in diameter, in the cisternae of the excess smooth endoplasmic reticulum (ER) of varying numbers of hepatocytes in 13 of 16 HB Ag carriers and in 4 of 9 patients with HB Ag-positive chronic hepatitis corresponding to cytoplasmic HB Ag-specific fluorescence. Direct immunoelectronmicroscopy using peroxidase-labeled HB Ab revealed that the intracisternal bodies and the surrounding membranes contain HB antigenic determinants. These bodies are an ultrastructural correlate of cytoplasmic HB Ag. It is suggested that they are virally coded coat material rather than the mature hepatitis B virus or its core. |
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Authors:
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M A Gerber; S Hadziyannis; C Vissoulis; F Schaffner; F Paronetto; H Popper |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The American journal of pathology Volume: 75 ISSN: 0002-9440 ISO Abbreviation: Am. J. Pathol. Publication Date: 1974 Jun |
Date Detail:
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Created Date: 1974-08-26 Completed Date: 1974-08-26 Revised Date: 2010-06-22 |
Medline Journal Info:
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Nlm Unique ID: 0370502 Medline TA: Am J Pathol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 489-502 Citation Subset: AIM; IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Antigens, Viral* Cell Nucleus Cytoplasm / immunology* Endoplasmic Reticulum Epitopes Fluorescent Antibody Technique* Hepatitis B / immunology Hepatitis B Antigens* Humans Inclusion Bodies Inclusion Bodies, Viral Liver / cytology, immunology* Microscopy, Electron* Organoids |
| Chemical | |
Reg. No./Substance:
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0/Antigens, Viral; 0/Epitopes; 0/Hepatitis B Antigens |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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