Document Detail


Electron microscopic visualization of trp operon expression in Salmonella typhimurium.
MedLine Citation:
PMID:  3895222     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Transcriptional activity of plasmids carrying wild-type and mutant trp operons was visualized in cell lysates of Salmonella typhimurium. Plasmid and transcription-unit sizes varied with the size of the cloned operon. Following 3-(3-indolyl)acrylic acid derepression, all operons of a particular type exhibited the same high level of transcriptional activity. An estimated 11-14 transcripts must be initiated each minute to maintain the 190-base-pair spacing of RNA polymerases observed on the promoter-proximal half of the wild-type trp operon. A decline in RNA polymerase density was observed on promoter-distal portions of cloned trp operons, which may be attributable to premature transcription termination accompanying translation inhibition due to indolylacrylic acid's interference with normal tryptophanyl-tRNA synthetase activity.
Authors:
S French; K Martin; T Patterson; R Bauerle; O L Miller
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  82     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  1985 Jul 
Date Detail:
Created Date:  1985-08-28     Completed Date:  1985-08-28     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  4638-42     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Chromosome Deletion
Chromosomes, Bacterial / ultrastructure
DNA, Recombinant / metabolism
DNA-Directed RNA Polymerases / metabolism
Genes, Bacterial*
Microscopy, Electron
Operon*
Plasmids*
Salmonella typhimurium / genetics*
Transcription, Genetic*
Grant Support
ID/Acronym/Agency:
GM07082/GM/NIGMS NIH HHS; GM21020/GM/NIGMS NIH HHS; GM26293/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/DNA, Recombinant; EC 2.7.7.6/DNA-Directed RNA Polymerases
Comments/Corrections

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