| Electron microscopic studies of endotoxins treated with alkaline and acid reagents. | |
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MedLine Citation:
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PMID: 6727716 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Endotoxins extracted from Salmonella minnesota wild strain and R mutants (Ra to Re) were treated with two different alkaline reagents. Treatment with diluted sodium hydroxide, which caused partial removal of O-ester linked fatty acids, changed the ultrastructures of endotoxins from an onion-like structure to monolayer particles (approximately 100 A in diameter) except for endotoxic glycolipids from Rd2 and Re mutants which showed mixed ultrastructures of untreated and treated endotoxins. Treatment with alkaline hydroxylamine, which caused liberation of all O-ester linked fatty acids, changed the ultrastructures of all endotoxins to monolayer particles. The results suggested that the ultrastructures of alkaline-treated endotoxins were dependent on the degree of their hydrophobicity. On the other hand, the micrographs of acid treated endotoxins did not show a constant structure because of the high hydrophobicity. |
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Authors:
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K Amano; K Fukushi |
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Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: Microbiology and immunology Volume: 28 ISSN: 0385-5600 ISO Abbreviation: Microbiol. Immunol. Publication Date: 1984 |
Date Detail:
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Created Date: 1984-07-11 Completed Date: 1984-07-11 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 7703966 Medline TA: Microbiol Immunol Country: JAPAN |
Other Details:
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Languages: eng Pagination: 161-8 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acetic Acids
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pharmacology Bacterial Toxins* / isolation & purification Chemical Phenomena Chemistry Endotoxins* / isolation & purification Enterotoxins* / isolation & purification Glycolipids* / isolation & purification Hydrochloric Acid / pharmacology Hydroxylamines / pharmacology Lipid A Lipopolysaccharides* / isolation & purification Microscopy, Electron Salmonella* Sodium Hydroxide / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Acetic Acids; 0/Bacterial Toxins; 0/Endotoxins; 0/Enterotoxins; 0/Glycolipids; 0/Hydroxylamines; 0/Lipid A; 0/Lipopolysaccharides; 0/salmonella toxin; 1310-73-2/Sodium Hydroxide; 7647-01-0/Hydrochloric Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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