Document Detail

Electron microscopic studies of endotoxins treated with alkaline and acid reagents.
MedLine Citation:
PMID:  6727716     Owner:  NLM     Status:  MEDLINE    
Endotoxins extracted from Salmonella minnesota wild strain and R mutants (Ra to Re) were treated with two different alkaline reagents. Treatment with diluted sodium hydroxide, which caused partial removal of O-ester linked fatty acids, changed the ultrastructures of endotoxins from an onion-like structure to monolayer particles (approximately 100 A in diameter) except for endotoxic glycolipids from Rd2 and Re mutants which showed mixed ultrastructures of untreated and treated endotoxins. Treatment with alkaline hydroxylamine, which caused liberation of all O-ester linked fatty acids, changed the ultrastructures of all endotoxins to monolayer particles. The results suggested that the ultrastructures of alkaline-treated endotoxins were dependent on the degree of their hydrophobicity. On the other hand, the micrographs of acid treated endotoxins did not show a constant structure because of the high hydrophobicity.
K Amano; K Fukushi
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Microbiology and immunology     Volume:  28     ISSN:  0385-5600     ISO Abbreviation:  Microbiol. Immunol.     Publication Date:  1984  
Date Detail:
Created Date:  1984-07-11     Completed Date:  1984-07-11     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  7703966     Medline TA:  Microbiol Immunol     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  161-8     Citation Subset:  IM    
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MeSH Terms
Acetic Acids / pharmacology
Bacterial Toxins* / isolation & purification
Chemical Phenomena
Endotoxins* / isolation & purification
Enterotoxins* / isolation & purification
Glycolipids* / isolation & purification
Hydrochloric Acid / pharmacology
Hydroxylamines / pharmacology
Lipid A
Lipopolysaccharides* / isolation & purification
Microscopy, Electron
Sodium Hydroxide / pharmacology
Reg. No./Substance:
0/Acetic Acids; 0/Bacterial Toxins; 0/Endotoxins; 0/Enterotoxins; 0/Glycolipids; 0/Hydroxylamines; 0/Lipid A; 0/Lipopolysaccharides; 0/salmonella toxin; 1310-73-2/Sodium Hydroxide; 7647-01-0/Hydrochloric Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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