| Electron-microscopic and hydrodynamic characterization of recombinant apolipoprotein (a) and its association with LDL. | |
| | |
MedLine Citation:
|
PMID: 8187249 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
A recombinant apo(a) containing 17 kringle 4 domains as well as the kringle 5 and protease domains of apo(a) was characterized by hydrodynamic studies and electron microscopy. Recombinant apo(a) is a monomer in solution with a molecular weight of 325,000 by sedimentation equilibrium and 320,000 by sedimentation and diffusion, and it is a highly asymmetric molecule with a frictional ratio of 2.2. In the electron microscope recombinant apo(a) is visualized as a flexible chain of domains approximately 800 A long. Sedimentation velocity studies also demonstrate that when it is mixed with LDL, recombinant apo(a) reversibly forms an Lp(a)-like complex with a 1:1 stoichiometry; moreover, complex formation is inhibited by 6-amino hexanoic acid. Hydrodynamic modeling and electron microscopy suggest that only a small portion of the r-apo(a) molecule interacts with the LDL and the rest of the chain extends into solution. Preliminary studies indicate that recombinant apo(a) also binds mouse LDL. |
| | |
Authors:
|
M L Phillips; A V Lembertas; V N Schumaker; R M Lawn; S J Shire; T F Zioncheck |
Related Documents
:
|
22358319 - Electrode kinetics at carbon electrodes and the density of electronic states. 1948629 - Secretory component of cystic craniopharyngiomas: a mucino-histochemical and electron-m... 7999639 - Erythropoiesis in mouse omental milky spots induced by erythropoietin: light and electr... 18396129 - Complex i: a complex gateway to the powerhouse. 6366569 - Ultrastructural features of phagocytosis and intracellular killing of candida albicans ... 23015459 - Multielectron-transfer templates via consecutive two-electron transformations: iron-sul... 21230879 - Quantum discord and the geometry of bell-diagonal states. 17444719 - Geometry optimization based on linear response free energy with quantum mechanical/mole... 22642669 - Enhanced cu2s/cds coaxial nanowire solar cells by piezo-phototronic effect. |
Publication Detail:
|
Type: In Vitro; Journal Article |
Journal Detail:
|
Title: Chemistry and physics of lipids Volume: 67-68 ISSN: 0009-3084 ISO Abbreviation: Chem. Phys. Lipids Publication Date: 1994 Jan |
Date Detail:
|
Created Date: 1994-06-23 Completed Date: 1994-06-23 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 0067206 Medline TA: Chem Phys Lipids Country: IRELAND |
Other Details:
|
Languages: eng Pagination: 91-7 Citation Subset: IM |
Affiliation:
|
Department of Chemistry and Biochemistry, University of California, Los Angeles 90024. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Apolipoproteins / chemistry, metabolism*, ultrastructure* Apoprotein(a) Humans Kringles Lipoprotein(a)* Lipoproteins, LDL / metabolism* Mice Microscopy, Electron Molecular Weight Protein Binding Protein Conformation Recombinant Proteins / chemistry, metabolism, ultrastructure Solutions Ultracentrifugation |
| Chemical | |
Reg. No./Substance:
|
0/Apolipoproteins; 0/Lipoprotein(a); 0/Lipoproteins, LDL; 0/Recombinant Proteins; 0/Solutions; EC 3.4.21.-/Apoprotein(a) |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Lp(a) lipoprotein: an overview.
Next Document: Primary structure of apoB-100.