Document Detail

Electron-microscopic cytochemical localization of diamine and polyamine oxidases in pea and maize tissues.
MedLine Citation:
PMID:  11538270     Owner:  NASA     Status:  MEDLINE    
An electron-microscopic cytochemical method was used to localize diamine oxidase (DAO) in pea and polyamine oxidase (PAO) in maize (Zea mays L.). The method, based on the precipitation of amine-oxidase-generated H2O2 by CeCl3, was shown to be specific for DAO and PAO and permitted their localization in plant tissues with a high degree of resolution. Both enzymes are localized exclusively in the cell wall. Both DAO- and PAO-activity staining is most intense in the middle lamellar region of the wall and in cells exhibiting highly lignified walls. The oxidases could provide H2O2 for peroxidase-mediated cross-linking reactions in the cell wall and may, in this capacity, play a role in the regulation of plant growth.
R D Slocum; M J Furey
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Planta     Volume:  183     ISSN:  0032-0935     ISO Abbreviation:  Planta     Publication Date:  1991 Feb 
Date Detail:
Created Date:  1996-03-25     Completed Date:  1996-03-25     Revised Date:  2011-11-02    
Medline Journal Info:
Nlm Unique ID:  1250576     Medline TA:  Planta     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  443-50     Citation Subset:  S    
Department of Biology, Williams College, Williamstown, MA 01267, USA.
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MeSH Terms
Amine Oxidase (Copper-Containing) / analysis*,  metabolism,  physiology
Cell Wall / enzymology*,  ultrastructure
Enzyme Inhibitors
Hydrogen Peroxide / metabolism
Microscopy, Electron
Oxidoreductases Acting on CH-NH Group Donors / analysis*,  metabolism,  physiology
Peas / enzymology*,  ultrastructure
Substrate Specificity
Zea mays / enzymology*,  ultrastructure
Reg. No./Substance:
0/Enzyme Inhibitors; 0/Guanidines; 0/Hydrazines; 0/Oxidants; 109-84-2/2-hydroxyethylhydrazine; 61-82-5/Amitrole; 7440-45-1/Cerium; 7722-84-1/Hydrogen Peroxide; 7790-86-5/cerous chloride; 79-17-4/pimagedine; EC Oxidase (Copper-Containing); EC 1.5.-/Oxidoreductases Acting on CH-NH Group Donors; EC 1.5.3.-/polyamine oxidase
R D Slocum / Williams Col, Williamstown, MA, Dept Biol

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