| Electron-microscopic cytochemical localization of diamine and polyamine oxidases in pea and maize tissues. | |
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MedLine Citation:
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PMID: 11538270 Owner: NASA Status: MEDLINE |
Abstract/OtherAbstract:
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An electron-microscopic cytochemical method was used to localize diamine oxidase (DAO) in pea and polyamine oxidase (PAO) in maize (Zea mays L.). The method, based on the precipitation of amine-oxidase-generated H2O2 by CeCl3, was shown to be specific for DAO and PAO and permitted their localization in plant tissues with a high degree of resolution. Both enzymes are localized exclusively in the cell wall. Both DAO- and PAO-activity staining is most intense in the middle lamellar region of the wall and in cells exhibiting highly lignified walls. The oxidases could provide H2O2 for peroxidase-mediated cross-linking reactions in the cell wall and may, in this capacity, play a role in the regulation of plant growth. |
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Authors:
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R D Slocum; M J Furey |
Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Planta Volume: 183 ISSN: 0032-0935 ISO Abbreviation: Planta Publication Date: 1991 Feb |
Date Detail:
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Created Date: 1996-03-25 Completed Date: 1996-03-25 Revised Date: 2011-11-02 |
Medline Journal Info:
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Nlm Unique ID: 1250576 Medline TA: Planta Country: GERMANY |
Other Details:
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Languages: eng Pagination: 443-50 Citation Subset: S |
Affiliation:
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Department of Biology, Williams College, Williamstown, MA 01267, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amine Oxidase (Copper-Containing)
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analysis*,
metabolism,
physiology Amitrole Cell Wall / enzymology*, ultrastructure Cerium Enzyme Inhibitors Guanidines Histocytochemistry Hydrazines Hydrogen Peroxide / metabolism Microscopy, Electron Oxidants Oxidoreductases Acting on CH-NH Group Donors / analysis*, metabolism, physiology Peas / enzymology*, ultrastructure Substrate Specificity Zea mays / enzymology*, ultrastructure |
| Chemical | |
Reg. No./Substance:
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0/Enzyme Inhibitors; 0/Guanidines; 0/Hydrazines; 0/Oxidants; 109-84-2/2-hydroxyethylhydrazine; 61-82-5/Amitrole; 7440-45-1/Cerium; 7722-84-1/Hydrogen Peroxide; 7790-86-5/cerous chloride; 79-17-4/pimagedine; EC 1.4.3.6/Amine Oxidase (Copper-Containing); EC 1.5.-/Oxidoreductases Acting on CH-NH Group Donors; EC 1.5.3.-/polyamine oxidase |
| Investigator | |
Investigator/Affiliation:
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R D Slocum / Williams Col, Williamstown, MA, Dept Biol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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