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Electron microscope autoradiographic study of intestinal absorption of decanoic and octanoic acids in the rat.
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MedLine Citation:
PMID:  1127017     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Intestinal absorption of [3H]octanoic acid and [3H]decanoic acid was investigated in the rat by electron microscope autoradiography. The common duct (bile and pancreatic common duct) of the rats was diverted and a loop of the duodenum was cannulated 24 h later. The lipid mixture to be investigated was introduced into each experimental loop, and after 15 min or less the loop was removed. One part of each loop was used to determine the distribution of radioactivity in different lipid fractions, and an autoradiographic study was performed on the other part of the loop. Radioactivity distribution studies confirmed that medium chain fatty acids are absorbed in their nonesterified form and established that these fatty acids are absorbed much more rapidly than oleic acid. Autoradiographic studies indicated that the medium chain fatty acids are taken up in a molecular or aggregate molecular form, leave the epithelial cells by way of the lateral plasma membrane, and are next found in the blood capillaries. Our results suggest that the Golgi complex does not play an important role in the absorption of unesterified fatty acids.
Authors:
H Carlier; J Bezard
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of cell biology     Volume:  65     ISSN:  0021-9525     ISO Abbreviation:  J. Cell Biol.     Publication Date:  1975 May 
Date Detail:
Created Date:  1975-07-23     Completed Date:  1975-07-23     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0375356     Medline TA:  J Cell Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  383-97     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Autoradiography / methods
Caprylates / metabolism*
Cell Membrane / metabolism
Chromatography, Gas
Cytoplasm / metabolism
Decanoic Acids / metabolism*
Fatty Acids / analysis
Intestinal Absorption*
Intestinal Mucosa / analysis
Lipids / analysis
Male
Microscopy, Electron
Octanoic Acids / metabolism*
Oleic Acids / metabolism
Rats
Tritium
Chemical
Reg. No./Substance:
0/Caprylates; 0/Decanoic Acids; 0/Fatty Acids; 0/Lipids; 0/Octanoic Acids; 0/Oleic Acids; 10028-17-8/Tritium
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Full Text
Journal Information
Journal ID (nlm-ta): J Cell Biol
ISSN: 0021-9525
ISSN: 1540-8140
Publisher: The Rockefeller University Press
Article Information
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Print publication date: Day: 1 Month: 5 Year: 1975
Volume: 65 Issue: 2
First Page: 383 Last Page: 397
ID: 2109422
Publisher Id: 75151743
PubMed Id: 1127017

Electron microscope autoradiographic study of intestinal absorption of decanoic and octanoic acids in the rat


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