Document Detail


Electron cytochemical study of carbohydrate components in different types of cultured glial cells of snail Helix pomatia.
MedLine Citation:
PMID:  7914854     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Using a variety of colloidal gold-labelled lectins with different sugar specificities, the structure and topography of carbohydrate determinants of the surface membrane of in vitro cultured glial and nerve cells of the snail Helix pomatia have been electron cytochemically studied. Heterogeneity of carbohydrate pools among different types of glial cells and between glial and nerve cells was established. It was found that satellite glial cells having the ultrastructural signs of cells with high metabolic level (type II cells) selectively bind GNA which is specific to terminal alpha-D-mannose residues and do not bind other mannose-specific lectins, Con A and LCA. GNA determinants are absent in satellite type I glial cells, fibrous glial cells, microglia and neurons. It has been found that glial cells (satellite type I and II glial cells, filamentous glial cells and microglial cells) do not bind PVA and LABA. LTA did not bind to any glial cells and binds weakly to neurons. Con A and WGA determinants which are abundant on the neurons are completely absent on satellite type II glial cells but present on satellite type I glial cells and filamentous glial cells. Microglial cells contain Con A and LCA determinants and the density of PNA determinants on these cells is the highest compared to other types of glial cells or neurons. It is concluded that some lectin determinants (for RCA-1, PNA, LPA) are present on all types of glial cells, while another determinant (GNA) is specific for a definite type of glial cells and can serve as a marker of these cells. The role of specific carbohydrate determinants in the functioning of a neuron-glial complex is discussed.
Authors:
L M Koval; N I Kononenko; M D Lutsik; E N Yavorskaya
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Comparative biochemistry and physiology. Comparative physiology     Volume:  108     ISSN:  -     ISO Abbreviation:  Comp. Biochem. Physiol. Comp. Physiol.     Publication Date:    1994 Jun-Jul
Date Detail:
Created Date:  1994-09-19     Completed Date:  1994-09-19     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  9441449     Medline TA:  Comp Biochem Physiol Comp Physiol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  195-212     Citation Subset:  IM    
Affiliation:
Laboratory of Neurobiology, A.A. Bogomoletz Institute of Physiology, Ukranian Academy of Sciences, Kiev.
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MeSH Terms
Descriptor/Qualifier:
Animals
Carbohydrate Sequence
Carbohydrates / chemistry*
Cells, Cultured
Gold Colloid
Helix (Snails) / physiology*
Histocytochemistry
Lectins
Microscopy, Electron
Molecular Sequence Data
Neuroglia / chemistry*,  ultrastructure
Neurons / chemistry,  ultrastructure
Chemical
Reg. No./Substance:
0/Carbohydrates; 0/Gold Colloid; 0/Lectins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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