Document Detail

Electron Transfer Dissociation of Oligonucleotide Cations.
MedLine Citation:
PMID:  20161288     Owner:  NLM     Status:  Publisher    
Electron transfer dissociation (ETD) of multi-protonated 6 - 20-mer oligonucleotides and 12- and 14-mer duplexes is compared to collision activated dissociation (CAD). ETD causes efficient charge reduction of the multi-protonated oligonucleotides in addition to limited backbone cleavages to yield sequence ions of low abundance. Subsequent CAD of the charge-reduced oligonucleotides formed upon electron transfer, in a net process termed electron transfer collision activated dissociation (ETcaD), results in rich fragmentation in terms of w, a, z, and d products, with a marked decrease in the abundance of base loss ions and internal fragments. Complete sequencing was possible for nearly all oligonucleotides studied. ETcaD of an oligonucleotide duplex resulted in specific backbone cleavages, with conservation of weaker non-covalent bonds.
Suncerae I Smith; Jennifer S Brodbelt
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Publication Detail:
Journal Detail:
Title:  International journal of mass spectrometry     Volume:  283     ISSN:  1387-3806     ISO Abbreviation:  -     Publication Date:  2009 Jun 
Date Detail:
Created Date:  2010-7-13     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101137096     Medline TA:  Int J Mass Spectrom     Country:  -    
Other Details:
Languages:  ENG     Pagination:  85-93     Citation Subset:  -    
Department of Chemistry and Biochemistry, University of Texas at Austin, Austin, TX 78712.
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Grant Support
R01 GM065956-04A1//NIGMS NIH HHS

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