| An electron spin resonance study for real-time detection of ascorbyl free radicals after addition of dimethyl sulfoxide in murine hippocampus or plasma during kainic acid-induced seizures. | |
| | |
MedLine Citation:
|
PMID: 20336368 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Electron spin resonance (ESR)-silent ascorbate solutions generate a detectable, likely concentration-dependent signal of ascorbyl free radicals (AFR) immediately upon addition of a molar excess of dimethyl sulfoxide (DMSO). We aimed to perform quantitative ESR analysis of AFR in real time after addition of DMSO (AFR/DMSO) to evaluate ascorbate concentrations in fresh hippocampus or plasma following systemic administration of kainate in mice. Use of a special tissue-type quartz cell allowed immediate detection of AFR/DMSO ESR spectra in fresh tissues from mice. AFR/DMSO content was increased significantly in fresh hippocampus or plasma obtained during kainate-induced seizures of mice, reaching maximum levels at 90 min after intraperitoneal administration of 50 mg/kg kainic acid. This suggests that oxidative injury of the hippocampus resulted from the accumulation of large amounts of ascorbic acid in the brain after kainic acid administration. AFR/DMSO content measured on an ESR spectrometer can be used for real-time evaluation of ascorbate content in fresh tissue. Due to the simplicity, good performance, low cost and real-time monitoring of ascorbate, this method may be applied to clinical research and treatment in the future. |
| | |
Authors:
|
Shigekiyo Matsumoto; Chihiro Shingu; Hironori Koga; Satoshi Hagiwara; Hideo Iwasaka; Takayuki Noguchi; Isao Yokoi |
Related Documents
:
|
6266808 - Corticotropin-releasing factors (crf) secreted by the rat median eminence in vitro in t... 3119508 - Difference in serum ascorbate in two species of callithricidae. 4596758 - Form of selenium in selenite enrichment media for isolation of salmonellae. 444558 - Stimulation of thiamine diphosphatase activity by ascorbic acid in rat brain microsomes. 22063688 - Effects of chlorine dioxide, cetylpyridinium chloride, lactic acid and trisodium phosph... 31628 - Dehydration of 2-hydroxy(ethoxy)-2-aryl-n.n-penta(tetra) methylenemorpholinium bromides. |
Publication Detail:
|
Type: Journal Article Date: 2010-03-25 |
Journal Detail:
|
Title: Neurochemical research Volume: 35 ISSN: 1573-6903 ISO Abbreviation: Neurochem. Res. Publication Date: 2010 Jul |
Date Detail:
|
Created Date: 2010-06-04 Completed Date: 2010-08-20 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 7613461 Medline TA: Neurochem Res Country: United States |
Other Details:
|
Languages: eng Pagination: 1010-6 Citation Subset: IM |
Affiliation:
|
Department of Anaesthesiology and Intensive Care Medicine, Oita University Faculty of Medicine, 1-1 Idaigaoka-Hasamamachi-Yufu, City-Oita, 879-5593, Japan. sigekiyo@med.oita-u.ac.jp |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Ascorbic Acid / blood, metabolism* Dimethyl Sulfoxide / pharmacology* Electron Spin Resonance Spectroscopy Free Radicals / blood, metabolism Hippocampus / metabolism* Kainic Acid* Male Mice Seizures / blood, chemically induced, metabolism* Specimen Handling |
| Chemical | |
Reg. No./Substance:
|
0/Free Radicals; 487-79-6/Kainic Acid; 50-81-7/Ascorbic Acid; 67-68-5/Dimethyl Sulfoxide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Pre and Post Natal Exposure of Fluoride Induced Oxidative Macromolecular Alterations in Developing C...
Next Document: A novel regiospecific cascade synthesis of sulfonamide derivatives from N-(2-polychloroethyl)sulfona...